Detailed information of TA system TA116396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4893766..4894433
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: VapC1
• Uniprot ID: -
• Locus tag: ELR50_RS21735
• Protein ID: WP_269962295.1
• Coordinates: 4893766..4894176 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: VapB1
• Uniprot ID: W5ISS6
• Locus tag: ELR50_RS21740
• Protein ID: WP_009613156.1
• Coordinates: 4894173..4894433 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS21715 (ELR50_21655)	4889877..4890410	+	534	WP_009613171.1	(2Fe-2S)-binding protein	-
ELR50_RS21720 (ELR50_21660)	4890413..4891648	+	1236	WP_269962292.1	cytochrome c	-
ELR50_RS21725 (ELR50_21665)	4891650..4892618	+	969	WP_269962293.1	XdhC family protein	-
ELR50_RS21730 (ELR50_21670)	4892615..4893175	+	561	WP_269962294.1	nucleotidyltransferase family protein	-
ELR50_RS21735 (ELR50_21675)	4893766..4894176	-	411	WP_269962295.1	type II toxin-antitoxin system VapC family toxin	Toxin
ELR50_RS21740 (ELR50_21680)	4894173..4894433	-	261	WP_009613156.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
ELR50_RS21745 (ELR50_21685)	4894516..4895640	-	1125	WP_269964278.1	class I SAM-dependent methyltransferase	-
ELR50_RS21750 (ELR50_21690)	4895769..4896545	+	777	WP_043268867.1	ferredoxin--NADP reductase	-
ELR50_RS21755 (ELR50_21695)	4896737..4898290	+	1554	WP_269962296.1	TerC family protein	-
ELR50_RS21760 (ELR50_21700)	4898308..4898721	-	414	WP_043268871.1	large-conductance mechanosensitive channel protein MscL	-
ELR50_RS21765 (ELR50_21705)	4898912..4899154	+	243	WP_269962297.1	YdcH family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• SpoVT-AbrB domain (11-49)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15501.85 Da
• Isoelectric Point: 7.7739

>T116396 WP_269962295.1 NZ_CP034688:c4894176-4893766 [Pseudomonas citronellolis]
VKYLLDTNILIYLLKNRPESVARRVNALPADAGLCMSFFTYAELLKGAERSTRRSEVLRQLERLTRQVPVVYDGTPRLCE
HYATQFTRLKLAGTPIGANDLWIACHALALEATLVTHNTREFERIDGLRLEDWAAE

Nucleotide

• Length: 411 bp

>T116396 NZ_CP034688:c4894176-4893766 [Pseudomonas citronellolis]
GTGAAATACCTGCTCGACACCAATATCCTCATCTACCTGCTGAAGAACCGCCCGGAATCCGTGGCCCGACGGGTCAACGC
CCTGCCCGCCGACGCCGGCCTGTGCATGTCCTTCTTCACCTACGCCGAGTTGCTCAAGGGCGCCGAACGCAGCACCCGGC
GCAGCGAGGTCCTGCGCCAGCTGGAGCGCCTGACGCGCCAGGTGCCGGTGGTCTACGACGGCACTCCGCGCCTGTGCGAG
CACTACGCGACGCAGTTCACCCGCCTCAAGCTGGCCGGCACGCCGATCGGCGCCAACGACCTGTGGATCGCCTGCCACGC
CCTGGCGCTGGAGGCGACCCTGGTGACGCACAACACCCGCGAGTTCGAACGCATCGACGGGCTACGCCTGGAAGACTGGG
CGGCCGAGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9789.91 Da
• Isoelectric Point: 4.2182

>AT116396 WP_009613156.1 NZ_CP034688:c4894433-4894173 [Pseudomonas citronellolis]
MSSPTLPSRVFMNGNSQAVRIPQEFRLDASRVEIRRQANGDLVIHPLPEDRGEALLQALSGFDDEFIEALEEGQREQPPM
QEREEL

Nucleotide

• Length: 261 bp

>AT116396 NZ_CP034688:c4894433-4894173 [Pseudomonas citronellolis]
ATGTCCTCGCCCACACTCCCCAGCCGCGTCTTCATGAACGGCAACAGCCAGGCGGTGCGCATTCCCCAGGAGTTCCGCCT
GGACGCCAGCCGCGTAGAAATCCGCCGCCAGGCCAATGGCGACCTGGTCATCCACCCATTGCCCGAGGACCGCGGCGAGG
CGTTGCTGCAGGCGCTGTCCGGCTTCGACGACGAGTTCATCGAAGCACTGGAAGAAGGGCAGCGCGAACAGCCGCCGATG
CAGGAGCGGGAAGAGCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A5D6X2