Detailed information of TA system TA116393

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 3306085..3306704
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: W5J3G4
• Locus tag: ELR50_RS14850
• Protein ID: WP_009615522.1
• Coordinates: 3306522..3306704 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: ELR50_RS14845
• Protein ID: WP_043319209.1
• Coordinates: 3306085..3306489 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS14820 (ELR50_14735)	3302020..3303726	+	1707	WP_269964209.1	group II intron reverse transcriptase/maturase	-
ELR50_RS14830 (ELR50_14745)	3304092..3305252	+	1161	WP_269964210.1	Fic family protein	-
ELR50_RS14835 (ELR50_14750)	3305257..3305682	-	426	WP_269964261.1	type II toxin-antitoxin system VapC family toxin	-
ELR50_RS14840 (ELR50_14755)	3305682..3305936	-	255	WP_269964211.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ELR50_RS14845 (ELR50_14760)	3306085..3306489	-	405	WP_043319209.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ELR50_RS14850 (ELR50_14765)	3306522..3306704	-	183	WP_009615522.1	type II toxin-antitoxin system HicA family toxin	Toxin
ELR50_RS14855 (ELR50_14770)	3307163..3307651	+	489	WP_064581809.1	BRO family protein	-
ELR50_RS14860 (ELR50_14775)	3307966..3308124	-	159	WP_009615518.1	YqaE/Pmp3 family membrane protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3292962..3307651	14689

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6962.13 Da
• Isoelectric Point: 12.2993

>T116393 WP_009615522.1 NZ_CP034688:c3306704-3306522 [Pseudomonas citronellolis]
MRSREIMELIRADGWFLVEVKGSHHQFRHFTKKGRVTVPHPRSNLPIGTVNSILRQAGLR

Nucleotide

• Length: 183 bp

>T116393 NZ_CP034688:c3306704-3306522 [Pseudomonas citronellolis]
ATGCGCAGTCGAGAAATCATGGAGTTGATCCGGGCGGATGGCTGGTTCCTGGTTGAGGTGAAAGGCAGTCATCACCAGTT
CCGGCATTTCACCAAGAAGGGGCGGGTGACGGTTCCCCACCCCAGGAGCAACCTGCCGATCGGGACCGTGAACAGCATTC
TGCGCCAGGCCGGCCTGCGATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14505.21 Da
• Isoelectric Point: 4.5714

>AT116393 WP_043319209.1 NZ_CP034688:c3306489-3306085 [Pseudomonas citronellolis]
MKFPVVLHKDADSDYGVTIPDVPGCFSAGGTVSQALENVQEALALHFEGLVADGETLPQAQEVDTHMGNPDYAGGVWAVV
DFDVTPYLGKAVRFNASLPENLLQRIDERVKRDHRYASRSGFLATAALRELSSS

Nucleotide

• Length: 405 bp

>AT116393 NZ_CP034688:c3306489-3306085 [Pseudomonas citronellolis]
ATGAAATTCCCCGTGGTTCTGCACAAGGACGCCGACTCGGATTACGGCGTGACCATCCCTGACGTGCCTGGCTGCTTCTC
CGCCGGCGGCACCGTCAGCCAGGCGCTGGAAAACGTCCAGGAAGCCCTGGCCCTGCACTTCGAAGGCCTGGTGGCCGACG
GCGAGACGCTCCCGCAAGCCCAGGAAGTGGACACCCACATGGGCAACCCCGACTACGCCGGCGGTGTCTGGGCGGTGGTC
GACTTCGACGTCACCCCCTACCTGGGCAAGGCCGTGCGCTTCAACGCCAGCCTCCCGGAAAACCTCCTGCAACGCATCGA
CGAACGCGTGAAGCGCGACCATCGCTACGCCTCCCGCTCCGGCTTCCTGGCTACCGCCGCGCTGCGTGAGTTGTCTTCGA
GCTGA

Structures

Toxin

• AlphaFold DB: A0A127MLP4