Detailed information of TA system TA116390

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA(toxin)
• Location: 2852075..2852664
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: ELR50_RS12565
• Protein ID: WP_269964072.1
• Coordinates: 2852413..2852664 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A1A5D829
• Locus tag: ELR50_RS12560
• Protein ID: WP_043318088.1
• Coordinates: 2852075..2852416 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS12540 (ELR50_12465)	2848996..2849844	+	849	WP_269964070.1	carboxylating nicotinate-nucleotide diphosphorylase	-
ELR50_RS12545 (ELR50_12470)	2850143..2850394	+	252	WP_197886989.1	helix-turn-helix domain-containing protein	-
ELR50_RS12550 (ELR50_12475)	2850394..2851590	+	1197	WP_269964071.1	type II toxin-antitoxin system HipA family toxin	-
ELR50_RS12555 (ELR50_12480)	2851778..2851927	-	150	WP_237770692.1	DUF433 domain-containing protein	-
ELR50_RS12560 (ELR50_12485)	2852075..2852416	-	342	WP_043318088.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
ELR50_RS12565 (ELR50_12490)	2852413..2852664	-	252	WP_269964072.1	type II toxin-antitoxin system HicA family toxin	Toxin
ELR50_RS12570 (ELR50_12500)	2852911..2853984	-	1074	WP_269964073.1	diguanylate cyclase	-
ELR50_RS12575 (ELR50_12505)	2854304..2855518	+	1215	WP_269964074.1	site-specific integrase	-
ELR50_RS12580 (ELR50_12510)	2855515..2856210	-	696	WP_156060094.1	hypothetical protein	-
ELR50_RS12585 (ELR50_12515)	2856209..2856409	+	201	WP_043272284.1	helix-turn-helix domain-containing protein	-
ELR50_RS12590	2856406..2856534	+	129	WP_256204891.1	hypothetical protein	-
ELR50_RS12595 (ELR50_12520)	2856531..2856800	+	270	WP_269964075.1	hypothetical protein	-
ELR50_RS12600 (ELR50_12525)	2856797..2857099	+	303	WP_269964076.1	hypothetical protein	-
ELR50_RS12605 (ELR50_12530)	2857223..2857423	+	201	WP_043272281.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	pilB / pilC / xcpA/pilD	2850394..2870259	19865

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (20-76)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9593.04 Da
• Isoelectric Point: 9.6935

>T116390 WP_269964072.1 NZ_CP034688:c2852664-2852413 [Pseudomonas citronellolis]
MSRNEKLLAKLLNESASFTWQELVTLLRWLGYRQLEGSGSRVKFDNGDPQALINLHKPHPDNELKAYVRRQIIEHLKAGE
LIP

Nucleotide

• Length: 252 bp

>T116390 NZ_CP034688:c2852664-2852413 [Pseudomonas citronellolis]
ATGTCCAGGAACGAGAAACTGCTGGCCAAGCTACTCAACGAAAGCGCCAGCTTTACCTGGCAGGAGCTTGTGACACTGCT
GCGTTGGCTGGGCTACAGGCAGTTGGAGGGCAGCGGGAGCCGGGTCAAGTTCGACAACGGCGACCCGCAGGCGTTGATCA
ACCTTCACAAGCCGCACCCGGACAACGAGCTGAAAGCCTATGTCCGGCGCCAGATCATCGAGCACCTCAAGGCGGGGGAA
CTGATTCCGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12586.02 Da
• Isoelectric Point: 5.0823

>AT116390 WP_043318088.1 NZ_CP034688:c2852416-2852075 [Pseudomonas citronellolis]
VNNLLQYRGYYGSIEASTEDNCLFGKLLFIRALVNYEGETVAELQAAFREAVDDYLATCQAQGQEPEVPCKGSFNVRVGH
DLHLAASLAANRQHMSLNDLTRQALSEYLQHHG

Nucleotide

• Length: 342 bp

>AT116390 NZ_CP034688:c2852416-2852075 [Pseudomonas citronellolis]
GTGAACAACCTGCTGCAATACCGGGGCTATTACGGCTCCATCGAAGCCAGCACCGAGGACAACTGCCTGTTCGGCAAGCT
GCTCTTCATCCGCGCGCTGGTCAACTACGAAGGCGAGACGGTGGCCGAACTGCAGGCCGCTTTCCGCGAGGCGGTGGACG
ACTACCTGGCCACATGCCAGGCGCAGGGGCAGGAGCCGGAAGTGCCTTGTAAGGGATCGTTCAACGTGCGGGTCGGCCAT
GACTTGCATCTGGCAGCGAGCCTGGCAGCCAACCGGCAGCACATGAGCCTGAACGACCTCACCCGGCAGGCGCTGAGCGA
GTATTTGCAGCACCACGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1A5D829