Detailed information of TA system TA116389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PfiT-PfiA/ParE(toxin)
• Location: 2515583..2516191
• Replicon: chromosome
• Accession: NZ_CP034688
• Organism: Pseudomonas citronellolis strain WXP-4

Toxin (Protein)

• Gene name: PfiT
• Uniprot ID: -
• Locus tag: ELR50_RS10975
• Protein ID: WP_269963918.1
• Coordinates: 2515844..2516191 (+)
• Length: 116 a.a.

Antitoxin (Protein)

• Gene name: PfiA
• Uniprot ID: -
• Locus tag: ELR50_RS10970
• Protein ID: WP_081884705.1
• Coordinates: 2515583..2515834 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELR50_RS10950 (ELR50_10890)	2510783..2512024	-	1242	WP_269963916.1	aminotransferase class III-fold pyridoxal phosphate-dependent enzyme	-
ELR50_RS10955 (ELR50_10895)	2512087..2512992	-	906	WP_043272449.1	LysR family transcriptional regulator	-
ELR50_RS10960 (ELR50_10900)	2513049..2513585	-	537	WP_043272448.1	2,4'-dihydroxyacetophenone dioxygenase family protein	-
ELR50_RS10965 (ELR50_10905)	2513711..2515126	-	1416	WP_269963917.1	amino acid permease	-
ELR50_RS10970 (ELR50_10910)	2515583..2515834	+	252	WP_081884705.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ELR50_RS10975 (ELR50_10915)	2515844..2516191	+	348	WP_269963918.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ELR50_RS10980 (ELR50_10920)	2516419..2517400	-	982	Protein_2165	IS5 family transposase	-
ELR50_RS10985 (ELR50_10925)	2517559..2520000	-	2442	WP_269963919.1	DUF2235 domain-containing protein	-
ELR50_RS10990 (ELR50_10930)	2520021..2520578	-	558	WP_269963920.1	DUF3304 domain-containing protein	-
ELR50_RS10995	2520575..2520703	-	129	WP_269963921.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-100)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 116 a.a.
• Molecular weight: 12944.76 Da
• Isoelectric Point: 4.8327

>T116389 WP_269963918.1 NZ_CP034688:2515844-2516191 [Pseudomonas citronellolis]
MSKVVIRLTDTAEQSIEDQVHHLAQFHGSQAALQSVLSLLDEIEEKISLTPQGYPISQQASLLGVLNYRELNTGPYRVFY
ELHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIG

Nucleotide

• Length: 348 bp

>T116389 NZ_CP034688:2515844-2516191 [Pseudomonas citronellolis]
ATGTCCAAGGTCGTCATCCGTCTCACCGATACCGCAGAACAAAGCATCGAAGACCAAGTACACCACTTGGCTCAATTCCA
TGGTTCGCAGGCTGCACTCCAGTCAGTGCTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCTCTTACACCCCAAGGTT
ACCCTATCAGCCAGCAGGCAAGCCTTTTAGGGGTGTTGAACTATCGCGAACTCAATACCGGCCCTTATCGCGTCTTTTAC
GAACTACACGAAGAGCAAGGCGAGGTCGCGGTTATCCTGGTCTTACGCCAGAAGCAGAGTGTGGAACAGCAATTGATTCG
CTACTGCTTGGTGGGGCCTATCGGGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9500.82 Da
• Isoelectric Point: 8.4753

>AT116389 WP_081884705.1 NZ_CP034688:2515583-2515834 [Pseudomonas citronellolis]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVIESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFD
QPE

Nucleotide

• Length: 252 bp

>AT116389 NZ_CP034688:2515583-2515834 [Pseudomonas citronellolis]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGCCATGCGGCCGACCTGGATTTATCCGAGCCAATGGTCGTCACACAGAA
CGGTGTTCCTGCCTACGTGATTGAGTCATATGCAGAACGAAAGCAACGTGACGAAGCAATTGCGTTGGTGAAGCTGCTTG
CGATTGGCTCTCGTCAATACGCAGAAGGCAAACATCGCTCTGTAGATGATCTGAAAGCTCGTCTTTCCAGGAGGTTCGAT
CAACCAGAATAA