Detailed information of TA system TA116384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2213370..2214330
• Replicon: chromosome
• Accession: NZ_CP034686
• Organism: Bacillus sp. BD59S

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: EKQ63_RS13420
• Protein ID: WP_140168735.1
• Coordinates: 2213370..2214107 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: EKQ63_RS13425
• Protein ID: WP_000588712.1
• Coordinates: 2214205..2214330 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKQ63_RS13400	2209099..2209881	+	783	WP_087952600.1	class I SAM-dependent methyltransferase	-
EKQ63_RS13405	2210003..2211688	-	1686	WP_088328200.1	alpha-keto acid decarboxylase family protein	-
EKQ63_RS13410	2211795..2212277	+	483	WP_088328201.1	MarR family winged helix-turn-helix transcriptional regulator	-
EKQ63_RS13415	2212445..2213182	+	738	WP_087952606.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EKQ63_RS13420	2213370..2214107	+	738	WP_140168735.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EKQ63_RS13425	2214205..2214330	+	126	WP_000588712.1	hypothetical protein	Antitoxin
EKQ63_RS13430	2214406..2214582	+	177	WP_000852620.1	stage II sporulation protein SB	-
EKQ63_RS13435	2214601..2214990	-	390	WP_087952612.1	YxeA family protein	-
EKQ63_RS13440	2215207..2216676	+	1470	WP_087952614.1	beta-Ala-His dipeptidase	-
EKQ63_RS13445	2217266..2217730	-	465	WP_087952619.1	DUF5065 family protein	-
EKQ63_RS13450	2218184..2218843	+	660	WP_087952621.1	CatB-related O-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (9-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28415.26 Da
• Isoelectric Point: 8.2638

>T116384 WP_140168735.1 NZ_CP034686:2213370-2214107 [Bacillus sp. BD59S]
VISNIRIGLFILAIVFLLLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDVAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIMIPLEYIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFIK

Nucleotide

• Length: 738 bp

>T116384 NZ_CP034686:2213370-2214107 [Bacillus sp. BD59S]
GTGATCTCTAACATTCGAATCGGCTTATTTATTTTAGCAATCGTTTTTCTATTACTTGTTTTCTTCTATTGGAAAAACGA
AGAGTTGTACGAGGAGAAGAAGCAACGTATTCGAAAGACTTGGTATGGGTTGTTTATAATATCAGTCACCGTATATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTCACTGCAATGGTTATTTTCGTTGATGTTGCATTT
ATTTTAACACCTAATATTTCAGAAATATGGGGAGCGAAATTTAGCGATATCGGCAAGACGGTTCAATCGATAAAACGGTC
ATTAATTGCTTCTAAAGCAAGAGGAGAAATATACACAACAATTATTCAAAATGTCAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAGGAAGAATATACAAAAAGCTTAAACGTATTTTTAGATTCGTACGGGGAAAAGATTGGAGCAAAAATT
GTTGTATTTGAAGCGGCAAAGGAATTAAATACAAACTTTCGTGGTATCCGTTCTCAATTTAGTATTATGATTCCGTTAGA
GTACATTGAGCAATTGAATGAGCAGAAAGCAGTGCAAGTAGAAAATGTCGGAATTATACCAGCAAAAATAGTGAGTGATG
TTTTCATTGTTATTGACGGGAAGAAAAATAATCTTCAAGACCGGGATTTTGAAAATGTATATAATTTAACGATACATCAT
AGTTATTTTATTAAGTAG

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT116384 WP_000588712.1 NZ_CP034686:2214205-2214330 [Bacillus sp. BD59S]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT116384 NZ_CP034686:2214205-2214330 [Bacillus sp. BD59S]
GTGATAAAAGATTATGACAATGATTTTTTCAAAGAAGATAATGAAGATACAACTGATTTCTCTTTAATAAAAGGAGATAC
GTTGCAGCAAGTAGAAGAGTTAGAAGAAGAATTAAAAAGAAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1