Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 216078..216720 Replicon chromosome
Accession NZ_CP034686
Organism Bacillus sp. BD59S

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EKQ63_RS03105 Protein ID WP_000635963.1
Coordinates 216370..216720 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EKQ63_RS03100 Protein ID WP_000004570.1
Coordinates 216078..216365 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKQ63_RS03075 211241..212203 + 963 WP_087952199.1 UV DNA damage repair endonuclease UvsE -
EKQ63_RS03080 212372..212920 - 549 WP_087952198.1 rhomboid family intramembrane serine protease -
EKQ63_RS03085 213013..213372 + 360 WP_000635039.1 holo-ACP synthase -
EKQ63_RS03090 213529..214479 + 951 WP_143881751.1 outer membrane lipoprotein carrier protein LolA -
EKQ63_RS03095 214598..215767 + 1170 WP_087952195.1 alanine racemase -
EKQ63_RS03100 216078..216365 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EKQ63_RS03105 216370..216720 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EKQ63_RS03110 216788..218956 + 2169 WP_143880717.1 RNA-binding transcriptional accessory protein -
EKQ63_RS03115 219014..219130 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EKQ63_RS03120 219326..219784 + 459 WP_062822141.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T116383 WP_000635963.1 NZ_CP034686:216370-216720 [Bacillus sp. BD59S]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T116383 NZ_CP034686:216370-216720 [Bacillus sp. BD59S]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CTAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT116383 WP_000004570.1 NZ_CP034686:216078-216365 [Bacillus sp. BD59S]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT116383 NZ_CP034686:216078-216365 [Bacillus sp. BD59S]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAACTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References