Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 158022..158761 Replicon plasmid pD120-1_296kb
Accession NZ_CP034681
Organism Klebsiella quasipneumoniae strain D120-1

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag ELE18_RS27405 Protein ID WP_013087172.1
Coordinates 158276..158761 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag ELE18_RS27400 Protein ID WP_012540086.1
Coordinates 158022..158288 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ELE18_RS27370 153254..153790 - 537 WP_012540238.1 GNAT family N-acetyltransferase -
ELE18_RS27375 153915..155666 - 1752 WP_162869309.1 arsenical pump-driving ATPase -
ELE18_RS27380 155693..156054 - 362 Protein_163 arsenic metallochaperone ArsD family protein -
ELE18_RS27385 156130..156675 - 546 WP_064175019.1 sigma-70 family RNA polymerase sigma factor -
ELE18_RS27390 156684..157397 - 714 WP_012540184.1 arsenical resistance protein ArsH -
ELE18_RS27395 157399..157722 - 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
ELE18_RS27400 158022..158288 + 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
ELE18_RS27405 158276..158761 + 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
ELE18_RS27415 160133..160699 + 567 WP_110212193.1 HNH endonuclease -
ELE18_RS27420 160806..162542 - 1737 WP_064175054.1 hypothetical protein -
ELE18_RS27425 162734..163282 - 549 WP_023320124.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..296653 296653


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T116378 WP_013087172.1 NZ_CP034681:158276-158761 [Klebsiella quasipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T116378 NZ_CP034681:158276-158761 [Klebsiella quasipneumoniae]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT116378 WP_012540086.1 NZ_CP034681:158022-158288 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT116378 NZ_CP034681:158022-158288 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCCAAGGAATCACAGCGGGCGCTCATTGACGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAATGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References