Detailed information of TA system TA116373

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2471468..2473056
• Replicon: chromosome
• Accession: NZ_CP034678
• Organism: Klebsiella quasipneumoniae strain D120-1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: ELE18_RS12955
• Protein ID: WP_094311661.1
• Coordinates: 2471468..2472790 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2A5MFC9
• Locus tag: ELE18_RS12960
• Protein ID: WP_004205921.1
• Coordinates: 2472790..2473056 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELE18_RS12915	2466550..2466768	+	219	WP_002904808.1	ParD-like family protein	-
ELE18_RS12920	2466749..2467531	+	783	WP_049093365.1	type I methionyl aminopeptidase	-
ELE18_RS12925	2467800..2468915	+	1116	WP_049093366.1	serine hydrolase	-
ELE18_RS12930	2468902..2469246	+	345	WP_004205915.1	hypothetical protein	-
ELE18_RS12935	2469275..2469808	+	534	WP_053810394.1	hypothetical protein	-
ELE18_RS12940	2469811..2470017	+	207	WP_004205917.1	helix-turn-helix transcriptional regulator	-
ELE18_RS12950	2470159..2471451	+	1293	WP_023318214.1	glycoside hydrolase family 10 protein	-
ELE18_RS12955	2471468..2472790	-	1323	WP_094311661.1	type II toxin-antitoxin system HipA family toxin	Toxin
ELE18_RS12960	2472790..2473056	-	267	WP_004205921.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
ELE18_RS12965	2473339..2473665	-	327	WP_094311660.1	hydroxyisourate hydrolase	-
ELE18_RS12970	2473662..2474162	-	501	WP_017900378.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
ELE18_RS12975	2474140..2475519	-	1380	WP_017900377.1	purine permease	-
ELE18_RS12980	2475865..2477019	+	1155	WP_094333832.1	FAD-dependent urate hydroxylase HpxO	-
ELE18_RS12985	2477000..2477923	-	924	WP_008804925.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	blaOKP-B-3	mgtC / iroE	2293440..2541521	248081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49030.36 Da
• Isoelectric Point: 8.2655

>T116373 WP_094311661.1 NZ_CP034678:c2472790-2471468 [Klebsiella quasipneumoniae]
MATLTTWMNNARVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRERIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIINTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILGEFADRMP
QAIESARQTLPSDFSAHVWRAITENMLKLHARLQQGLLAG

Nucleotide

• Length: 1323 bp

>T116373 NZ_CP034678:c2472790-2471468 [Klebsiella quasipneumoniae]
ATGGCGACGCTCACCACCTGGATGAACAATGCGCGCGTGGGAACCTTAACCCGCCAGGCCAACGGGGCGCACAGTTTCCG
CTATGATGAGGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCGCTGCCGCTGCAGTATGGCAATATTACCG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGAGCGGATCGTCAGGCGCTATCAG
GCGCGTTCGAAACAGCCGTTCGATCTGCTGGCCGAGGTCGGGCGGGACAGCGTAGGGGCGGTGACTCTGCTGCCGCCGGG
GGAAGAGGCGCATCTTGCCGCGTTGCGCTGGCAGACGCTGGATGAGGCGCAGCTGACCGCTTTGCTGACCGCCTACCAGT
CGGATATTCCTCTCGGGATGGTGACCGGCCAGGACGATTTTCGTATCTCGGTGGCCGGCGCTCAGGAGAAAACCGCGCTG
CTGCGAATGGGCGAGCATTGGTGTATTCCGCAGGGCACCACCCCGACCACCCATATCATTAAACTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAACGAATATCTGTGCCTGGCGCTGGCCAGGGCGTTGG
GCCTGGCGGTGCCGGAGGCGGAGATTATCAACACGCCGCGGATCCGTGCGCTGGCGGTGACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGGCGCGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCATTCGGCATACCGTCGTCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCCAGTGAGGCCCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTGTTCCAGTGGCTGACCGGGGCAACGGACGGGCATGCGAAGAACTTCTCGCTCTTTTTGCTG
CCCGGCGGCAGCTACCGGCTCACGCCGTTCTACGACATTATTTCGGCATTTCCGGTGCTGGGCGGGACGGGACTGCATCT
GCGCGACCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGGAAAACGGAGATTAACGCTATCTATCCGCGTCATT
TTCTGGCGACCGCCAAAGCGGTTAACTTCCCCCGGGAGCAGATGCTGGCGATCCTGGGCGAGTTTGCCGACCGCATGCCG
CAGGCCATCGAAAGCGCCCGCCAAACGCTGCCGTCGGACTTTTCCGCCCACGTCTGGCGGGCCATCACGGAGAACATGCT
TAAACTGCACGCCCGCCTGCAGCAAGGGCTGCTGGCGGGATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.38 Da
• Isoelectric Point: 7.1997

>AT116373 WP_004205921.1 NZ_CP034678:c2473056-2472790 [Klebsiella quasipneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETAMIP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT116373 NZ_CP034678:c2473056-2472790 [Klebsiella quasipneumoniae]
ATGAATTCCCCGCAGATATACAGTCCGGTTCAGCTGGCCAATTACCTGAAACTGCTGCGCCAGCAAAATCACTGGACTCA
GGAGGCGCTGGCCAGCCGGATCGGCATTAAACAGGCGACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACCCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTAATGACCCTGAACGCCAAACCGGCAGAGACGGCGATGATTCCC
GATAACACCACGGATCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2A5MFC9