Detailed information of TA system TA116364

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 763104..763761
• Replicon: chromosome
• Accession: NZ_CP034678
• Organism: Klebsiella quasipneumoniae strain D120-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: A0A2A5MNX1
• Locus tag: ELE18_RS04420
• Protein ID: WP_004205323.1
• Coordinates: 763351..763761 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: ELE18_RS04415
• Protein ID: WP_127113158.1
• Coordinates: 763104..763370 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELE18_RS04390	758312..759745	-	1434	WP_094333723.1	6-phospho-beta-glucosidase BglA	-
ELE18_RS04395	759864..760592	-	729	WP_094333724.1	MurR/RpiR family transcriptional regulator	-
ELE18_RS04400	760643..760954	+	312	WP_094333725.1	N(4)-acetylcytidine aminohydrolase	-
ELE18_RS04405	761117..761776	+	660	WP_004205319.1	hemolysin III family protein	-
ELE18_RS04410	761875..762858	-	984	WP_017899798.1	tRNA-modifying protein YgfZ	-
ELE18_RS04415	763104..763370	+	267	WP_127113158.1	FAD assembly factor SdhE	Antitoxin
ELE18_RS04420	763351..763761	+	411	WP_004205323.1	protein YgfX	Toxin
ELE18_RS04425	763768..764289	-	522	WP_004205324.1	flavodoxin FldB	-
ELE18_RS04430	764390..765286	+	897	WP_004144729.1	site-specific tyrosine recombinase XerD	-
ELE18_RS04435	765309..766022	+	714	WP_004205326.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
ELE18_RS04440	766028..767761	+	1734	WP_094333727.1	single-stranded-DNA-specific exonuclease RecJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (8-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16035.83 Da
• Isoelectric Point: 11.4778

>T116364 WP_004205323.1 NZ_CP034678:763351-763761 [Klebsiella quasipneumoniae]
VVLWQSDLRISWRAQWFSLLLHGVVAALVLLVPWPLSYTPIWLLLLSLVVFDCVRSQRRIHARRGEIKLLTDSRLRWQNA
DWEILGTPWVINSGMLLRLRHVDTRRGQHLWLAADSMDAGEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T116364 NZ_CP034678:763351-763761 [Klebsiella quasipneumoniae]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCTCTTCTGCTCCACGGCGTTGTGGCGGC
GCTGGTGCTGCTGGTTCCCTGGCCGTTAAGCTACACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTTGACTGTG
TGCGCAGCCAGCGACGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGGCTGCGCTGGCAAAACGCC
GACTGGGAGATCCTCGGGACGCCGTGGGTCATCAACAGCGGAATGCTGCTGCGTTTGCGCCATGTCGATACCCGACGCGG
TCAGCATCTGTGGCTGGCGGCGGACAGCATGGATGCCGGAGAGTGGCGGGACCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10461.94 Da
• Isoelectric Point: 5.0965

>AT116364 WP_127113158.1 NZ_CP034678:763104-763370 [Klebsiella quasipneumoniae]
MDINNKAHIHWACRRGMRELDISIMPFFEYEYDTLSDADKQLFIRLLENDDPDLFNWLMNHGKPADAELQRMVTLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT116364 NZ_CP034678:763104-763370 [Klebsiella quasipneumoniae]
ATGGACATTAACAATAAAGCACATATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TATTCAACTGGCTGATGAACCATGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A2A5MNX1