Detailed information of TA system TA116363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 316530..317116
• Replicon: chromosome
• Accession: NZ_CP034678
• Organism: Klebsiella quasipneumoniae strain D120-1

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: ELE18_RS02055
• Protein ID: WP_002920800.1
• Coordinates: 316748..317116 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: ELE18_RS02050
• Protein ID: WP_048295677.1
• Coordinates: 316530..316751 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ELE18_RS02030	312687..313613	+	927	WP_127113126.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
ELE18_RS02035	313610..314887	+	1278	WP_008806971.1	branched chain amino acid ABC transporter permease LivM	-
ELE18_RS02040	314884..315651	+	768	WP_008806972.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
ELE18_RS02045	315653..316366	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
ELE18_RS02050	316530..316751	+	222	WP_048295677.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
ELE18_RS02055	316748..317116	+	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
ELE18_RS02060	317408..318724	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
ELE18_RS02065	318831..319718	+	888	WP_004202113.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
ELE18_RS02070	319715..320560	+	846	WP_004202115.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
ELE18_RS02075	320562..321632	+	1071	WP_017899977.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	313610..322369	8759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T116363 WP_002920800.1 NZ_CP034678:316748-317116 [Klebsiella quasipneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T116363 NZ_CP034678:316748-317116 [Klebsiella quasipneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCATGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCTGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8135.20 Da
• Isoelectric Point: 5.0637

>AT116363 WP_048295677.1 NZ_CP034678:316530-316751 [Klebsiella quasipneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGVEIRELADK

Nucleotide

• Length: 222 bp

>AT116363 NZ_CP034678:316530-316751 [Klebsiella quasipneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGTTGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1