Detailed information of TA system TA116362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YafQ-RelB
• Location: 801851..802391
• Replicon: chromosome
• Accession: NZ_CP034673
• Organism: Vibrio anguillarum strain J360

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A289GHH1
• Locus tag: DYL72_RS19965
• Protein ID: WP_069212181.1
• Coordinates: 802122..802391 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A233HK77
• Locus tag: DYL72_RS19960
• Protein ID: WP_019283191.1
• Coordinates: 801851..802129 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYL72_RS19920	797809..798216	-	408	WP_175418697.1	hypothetical protein	-
DYL72_RS19925	798265..798357	-	93	WP_140288197.1	DUF3265 domain-containing protein	-
DYL72_RS19930	798472..799440	+	969	WP_081245536.1	IS30 family transposase	-
DYL72_RS19935	799475..799945	-	471	WP_019282925.1	hypothetical protein	-
DYL72_RS19945	800148..800561	-	414	WP_019283386.1	RDD family protein	-
DYL72_RS19950	801348..801584	+	237	WP_013867767.1	hypothetical protein	-
DYL72_RS19955	801684..801794	-	111	WP_094164944.1	DUF3265 domain-containing protein	-
DYL72_RS19960	801851..802129	+	279	WP_019283191.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DYL72_RS19965	802122..802391	+	270	WP_069212181.1	type II toxin-antitoxin system YafQ family toxin	Toxin
DYL72_RS19975	803006..803326	+	321	WP_081245520.1	IS66 family insertion sequence hypothetical protein	-
DYL72_RS19980	803323..803676	+	354	WP_069212062.1	IS66 family insertion sequence element accessory protein TnpB	-
DYL72_RS19985	803736..805268	+	1533	WP_069212063.1	IS66-like element ISVa9 family transposase	-
DYL72_RS19990	805260..805421	-	162	WP_206499664.1	transposase	-
DYL72_RS19995	805434..806966	-	1533	WP_069211897.1	IS66-like element ISVa11 family transposase	-
DYL72_RS20000	807026..807379	-	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	776131..799440	23309
	-	inside	IScluster/Tn	-	-	778767..807955	29188
	-	inside	Integron	-	-	786908..802534	15626

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (3-84)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10236.89 Da
• Isoelectric Point: 8.9357

>T116362 WP_069212181.1 NZ_CP034673:802122-802391 [Vibrio anguillarum]
MYSLEYSTQFKKDFKKITKMPISDIIEVGNVISKLQRGEKLDPKNVDHPLTGGWVGFRDCHVKPDLVLIYRIFDGQLQLA
RIGSHSDLF

Nucleotide

• Length: 270 bp

>T116362 NZ_CP034673:802122-802391 [Vibrio anguillarum]
ATGTATAGTTTAGAATACTCAACACAATTCAAAAAAGACTTTAAAAAAATCACCAAAATGCCGATTTCGGACATCATTGA
AGTCGGCAACGTCATATCAAAGCTGCAAAGAGGCGAAAAACTCGATCCTAAAAATGTTGATCATCCTTTAACTGGCGGAT
GGGTTGGATTCCGAGACTGTCACGTTAAACCAGATCTAGTGCTCATTTATCGCATTTTTGATGGTCAACTCCAATTAGCT
AGAATTGGCTCTCATAGTGATTTGTTCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 9995.47 Da
• Isoelectric Point: 6.4761

>AT116362 WP_019283191.1 NZ_CP034673:801851-802129 [Vibrio anguillarum]
MRTEMLSTRIDHDTKVAFTSICDEMGLSTSQAIKIFAKAVINHGGIPFDLRVPQPNETTISAMNELVQGHGHKAESVGAM
LTELTEGKVKNV

Nucleotide

• Length: 279 bp

>AT116362 NZ_CP034673:801851-802129 [Vibrio anguillarum]
ATGAGAACAGAAATGCTAAGTACTCGCATAGACCATGATACCAAAGTTGCTTTTACTAGCATCTGTGACGAAATGGGTCT
TAGTACATCACAAGCTATTAAAATTTTTGCAAAAGCCGTGATTAATCATGGTGGTATTCCATTTGATTTACGTGTACCTC
AACCAAATGAAACAACGATTTCTGCAATGAATGAACTCGTTCAAGGTCATGGACATAAAGCCGAATCTGTTGGCGCTATG
CTTACTGAGCTAACTGAAGGCAAAGTTAAGAATGTATAG

Structures

Toxin

• AlphaFold DB: A0A289GHH1

Antitoxin

• AlphaFold DB: A0A233HK77