Detailed information of TA system TA116357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 608607..609166
• Replicon: chromosome
• Accession: NZ_CP034673
• Organism: Vibrio anguillarum strain J360

Toxin (Protein)

• Gene name: relE
• Uniprot ID: F7YRQ7
• Locus tag: DYL72_RS18715
• Protein ID: WP_010320663.1
• Coordinates: 608607..608885 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A233HRF0
• Locus tag: DYL72_RS18720
• Protein ID: WP_017040620.1
• Coordinates: 608882..609166 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYL72_RS18675	604103..604456	-	354	WP_019283195.1	IS66 family insertion sequence element accessory protein TnpB	-
DYL72_RS18680	604453..604773	-	321	WP_019283194.1	IS66 family insertion sequence hypothetical protein	-
DYL72_RS18685	604856..605149	-	294	Protein_529	helix-turn-helix domain-containing protein	-
DYL72_RS18690	605805..606590	-	786	WP_019283218.1	DUF4225 domain-containing protein	-
DYL72_RS18695	606583..607076	-	494	Protein_531	Hcp family type VI secretion system effector	-
DYL72_RS18705	607986..608465	+	480	WP_017045717.1	lecithin retinol acyltransferase family protein	-
DYL72_RS18715	608607..608885	-	279	WP_010320663.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
DYL72_RS18720	608882..609166	-	285	WP_017040620.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
DYL72_RS18725	609364..609869	+	506	Protein_535	lipocalin family protein	-
DYL72_RS18735	611325..611567	+	243	WP_095661368.1	DUF1289 domain-containing protein	-
DYL72_RS18740	611687..611917	-	231	WP_013868597.1	PAS factor family protein	-
DYL72_RS18750	612221..612508	+	288	WP_029388214.1	type II toxin-antitoxin system RelE/ParE family toxin	-
DYL72_RS18755	612519..612836	+	318	WP_001232701.1	HigA family addiction module antidote protein	-
DYL72_RS18765	612985..613401	+	417	WP_017045395.1	VOC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	610176..632066	21890
	-	inside	Integron	-	-	592798..625119	32321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (5-44)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10878.58 Da
• Isoelectric Point: 4.3430

>T116357 WP_010320663.1 NZ_CP034673:c608885-608607 [Vibrio anguillarum]
MILWEEESLNDREKIFEFLYDFNPDAAEKTDDIIEAKVENLLEQPLMGVQRDGIRGRLLIIPEISMIVSYWVEGSIIRIM
RVLHQKQKFPTD

Nucleotide

• Length: 279 bp

>T116357 NZ_CP034673:c608885-608607 [Vibrio anguillarum]
ATGATTTTATGGGAAGAAGAATCGCTAAATGATCGTGAGAAGATCTTTGAGTTTCTTTATGACTTTAACCCTGATGCGGC
TGAAAAAACTGATGACATTATTGAGGCTAAAGTAGAAAACTTACTCGAACAACCATTGATGGGTGTTCAACGGGATGGAA
TTCGAGGACGATTGCTCATTATTCCCGAAATCTCAATGATCGTTTCGTACTGGGTAGAAGGTTCAATTATTCGCATAATG
CGAGTTCTACACCAGAAGCAGAAATTCCCTACCGATTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10957.29 Da
• Isoelectric Point: 9.2173

>AT116357 WP_017040620.1 NZ_CP034673:c609166-608882 [Vibrio anguillarum]
MDTRIQFRVDDEIKRLAQQMAESQGRTLSDACRELTEQMAEQQRKTLSHDAWLTEQINLAFEKFDSGKSSFVEHNSAKAR
MAERKAKIRNRGQQ

Nucleotide

• Length: 285 bp

>AT116357 NZ_CP034673:c609166-608882 [Vibrio anguillarum]
ATGGACACTAGAATTCAATTTCGTGTTGATGACGAAATTAAACGTTTAGCCCAACAAATGGCTGAGAGCCAAGGCCGCAC
ACTCAGCGATGCTTGCCGTGAACTTACTGAACAAATGGCTGAGCAACAAAGAAAAACATTATCTCATGACGCTTGGTTAA
CTGAACAAATAAATCTAGCATTTGAGAAGTTTGACTCAGGAAAATCTTCATTTGTTGAACATAATTCAGCTAAAGCACGA
ATGGCTGAACGAAAGGCCAAAATTCGTAATCGAGGCCAGCAATGA

Structures

Toxin

• AlphaFold DB: A0A0F2IQF3

Antitoxin

• AlphaFold DB: A0A233HRF0