Detailed information of TA system TA116356

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /GNAT-DUF1778
• Location: 597592..598358
• Replicon: chromosome
• Accession: NZ_CP034673
• Organism: Vibrio anguillarum strain J360

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A290PTK1
• Locus tag: DYL72_RS18625
• Protein ID: WP_017045788.1
• Coordinates: 597592..598089 (-)
• Length: 166 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A290Q6J3
• Locus tag: DYL72_RS18630
• Protein ID: WP_019283269.1
• Coordinates: 598086..598358 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
DYL72_RS18580	592798..593313	+	516	WP_019283453.1	peptide deformylase	-
DYL72_RS18595	595104..595817	+	714	WP_019283452.1	Fic family protein	-
DYL72_RS18600	595814..595924	+	111	Protein_514	DUF3265 domain-containing protein	-
DYL72_RS18605	595974..596351	+	378	WP_019283451.1	hypothetical protein	-
DYL72_RS18615	596467..597435	+	969	WP_081245536.1	IS30 family transposase	-
DYL72_RS18620	597480..597569	+	90	Protein_517	hypothetical protein	-
DYL72_RS18625	597592..598089	-	498	WP_017045788.1	GNAT family N-acetyltransferase	Toxin
DYL72_RS18630	598086..598358	-	273	WP_019283269.1	DUF1778 domain-containing protein	Antitoxin
DYL72_RS18635	598398..598559	+	162	WP_019283270.1	DUF3265 domain-containing protein	-
DYL72_RS18645	599108..599527	+	420	WP_069212067.1	hypothetical protein	-
DYL72_RS18650	600668..601118	+	451	Protein_522	effector binding domain-containing protein	-
DYL72_RS18655	601262..601783	+	522	WP_019283291.1	GNAT family N-acetyltransferase	-
DYL72_RS18660	601934..602281	+	348	WP_019283292.1	hypothetical protein	-
DYL72_RS18665	602317..602478	-	162	Protein_525	IS30 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	551258..597435	46177
	-	inside	Integron	-	-	592798..625119	32321

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (86-152)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 166 a.a.
• Molecular weight: 18524.30 Da
• Isoelectric Point: 9.2813

>T116356 WP_017045788.1 NZ_CP034673:c598089-597592 [Vibrio anguillarum]
MMKTVLLDKDKHDRNRFNCGIDALNNYLKVMASQQAKKDNTRTFVLEDENNTSLIIGFYTLTMTPIDLKTLPDKLQKKHQ
SSTSGGLIARLAIDDSYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFKAFQDAENKLFITISDI
RASLG

Nucleotide

• Length: 498 bp

>T116356 NZ_CP034673:c598089-597592 [Vibrio anguillarum]
ATGATGAAAACGGTATTACTCGATAAAGATAAACACGACAGAAACCGATTCAACTGCGGAATTGATGCGCTTAATAACTA
TTTGAAAGTCATGGCAAGTCAGCAAGCTAAAAAAGATAATACCCGAACATTTGTTTTAGAGGATGAAAATAACACCTCAC
TTATCATCGGTTTTTACACTTTAACAATGACACCCATTGACCTAAAAACATTGCCTGATAAGTTACAGAAAAAGCATCAG
TCATCAACTTCTGGCGGACTCATTGCTCGCTTAGCTATCGATGATAGCTATAAAGGTAAAGGATTTGGCGAGTGGTTACT
TATCGATGCTCTACGAAAACTACTAGCAGCTAGTGATAGCGTGGCATTTCCCGTCGTTATTGTTGACGCAAAAGATGGCG
CCAAACATTTCTATGAGCGCTATGGTTTTAAAGCGTTTCAAGATGCAGAAAATAAGCTATTTATCACCATTTCAGATATT
AGGGCAAGTTTAGGCTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9917.47 Da
• Isoelectric Point: 9.8130

>AT116356 WP_019283269.1 NZ_CP034673:c598358-598086 [Vibrio anguillarum]
MATTLPRITARVDVDTQNLLTKAAALAGMSSINSFVLNAAIEKAKQVIEREQTLKLSQTDAILLMEALDKPTTLNQKLKT
AAERYETRTQ

Nucleotide

• Length: 273 bp

>AT116356 NZ_CP034673:c598358-598086 [Vibrio anguillarum]
ATGGCTACCACTTTACCTCGTATCACTGCGAGAGTTGATGTCGATACACAAAACTTATTAACCAAAGCGGCTGCTCTCGC
TGGCATGTCTAGTATTAACTCATTTGTACTCAATGCTGCAATCGAAAAAGCAAAGCAAGTGATTGAACGCGAACAAACAC
TAAAATTAAGCCAAACAGACGCCATTTTACTTATGGAAGCTCTCGATAAACCAACAACATTAAATCAAAAGCTTAAAACT
GCCGCCGAACGCTATGAGACCAGAACCCAATGA

Structures

Toxin

• AlphaFold DB: A0A290PTK1

Antitoxin

• AlphaFold DB: A0A290Q6J3