Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 422536..423137 Replicon plasmid unnamed
Accession NZ_CP034659
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag EKH78_RS24400 Protein ID WP_001216045.1
Coordinates 422536..422916 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EKH78_RS24405 Protein ID WP_001190712.1
Coordinates 422916..423137 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS24375 (EKH78_24720) 417976..419418 - 1443 WP_001472843.1 hypothetical protein -
EKH78_RS24380 (EKH78_24725) 419460..420653 - 1194 WP_000219607.1 hypothetical protein -
EKH78_RS24385 (EKH78_24730) 420740..421192 - 453 WP_016236875.1 hypothetical protein -
EKH78_RS24390 (EKH78_24735) 421281..422324 - 1044 WP_000648821.1 DUF968 domain-containing protein -
EKH78_RS24395 (EKH78_24740) 422352..422531 - 180 WP_000113018.1 hypothetical protein -
EKH78_RS24400 (EKH78_24745) 422536..422916 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EKH78_RS24405 (EKH78_24750) 422916..423137 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EKH78_RS24410 (EKH78_24755) 423320..424348 + 1029 Protein_448 type I restriction-modification system subunit M -
EKH78_RS24415 (EKH78_24760) 424405..425373 - 969 WP_000654812.1 IS5-like element IS903B family transposase -
EKH78_RS24420 (EKH78_24770) 425406..425942 + 537 Protein_450 N-6 DNA methylase -
EKH78_RS24425 (EKH78_24775) 425939..427144 + 1206 WP_001293319.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - mrkA / mrkB / mrkC / mrkD / mrkF 1..454520 454520
- flank IS/Tn - - 424405..425373 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T116344 WP_001216045.1 NZ_CP034659:c422916-422536 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T116344 NZ_CP034659:c422916-422536 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGCGCG
CTGCTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACTGTAGGCGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT116344 WP_001190712.1 NZ_CP034659:c423137-422916 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT116344 NZ_CP034659:c423137-422916 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGTCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References