Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | parDE/ParE-RHH |
| Location | 367853..368372 | Replicon | plasmid unnamed |
| Accession | NZ_CP034659 | ||
| Organism | Escherichia coli strain ATCC 98082 | ||
Toxin (Protein)
| Gene name | parE | Uniprot ID | A0A837F4A9 |
| Locus tag | EKH78_RS24115 | Protein ID | WP_000277490.1 |
| Coordinates | 368088..368372 (+) | Length | 95 a.a. |
Antitoxin (Protein)
| Gene name | parD | Uniprot ID | A0A6P1V8C3 |
| Locus tag | EKH78_RS24110 | Protein ID | WP_000124732.1 |
| Coordinates | 367853..368095 (+) | Length | 81 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKH78_RS24090 (EKH78_24410) | 363578..363688 | + | 111 | Protein_383 | recombinase family protein | - |
| EKH78_RS24095 (EKH78_24420) | 363750..364454 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS24100 (EKH78_24425) | 364516..366450 | + | 1935 | Protein_385 | Tn3 family transposase | - |
| EKH78_RS24105 (EKH78_24430) | 366468..367280 | + | 813 | WP_257606735.1 | plasmid replication initiator RepA | - |
| EKH78_RS24110 (EKH78_24435) | 367853..368095 | + | 243 | WP_000124732.1 | type II toxin-antitoxin system ParD family antitoxin | Antitoxin |
| EKH78_RS24115 (EKH78_24440) | 368088..368372 | + | 285 | WP_000277490.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| EKH78_RS24120 (EKH78_24445) | 368510..368602 | - | 93 | Protein_389 | plasmid partition protein ParG | - |
| EKH78_RS24845 | 368922..369140 | - | 219 | Protein_390 | IS110 family transposase | - |
| EKH78_RS24125 | 369141..369440 | - | 300 | Protein_391 | glycogen/starch synthase | - |
| EKH78_RS24130 (EKH78_24455) | 369440..369961 | - | 522 | Protein_392 | glucose-1-phosphate adenylyltransferase | - |
| EKH78_RS24135 (EKH78_24460) | 370024..370728 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS24140 (EKH78_24465) | 370738..371889 | + | 1152 | WP_212485024.1 | DUF2254 domain-containing protein | - |
| EKH78_RS24145 (EKH78_24470) | 372194..372373 | + | 180 | WP_230586338.1 | transposase | - |
| EKH78_RS24150 (EKH78_24475) | 372331..372612 | - | 282 | Protein_396 | IS6-like element IS26 family transposase | - |
| EKH78_RS24155 (EKH78_24480) | 372589..372930 | + | 342 | WP_001395492.1 | recombinase family protein | - |
| EKH78_RS24160 (EKH78_24485) | 372969..373343 | + | 375 | WP_000110556.1 | ArsA-related P-loop ATPase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | mrkA / mrkB / mrkC / mrkD / mrkF | 1..454520 | 454520 | |
| - | inside | IScluster/Tn | - | - | 364597..370728 | 6131 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 95 a.a. Molecular weight: 11005.52 Da Isoelectric Point: 6.2103
>T116343 WP_000277490.1 NZ_CP034659:368088-368372 [Escherichia coli]
VYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHTVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL
VYKLTERAADDFAGIYDYTLLKFGEAQADHYTDALEAFFETLAGMPDMGRDYHTVPGVMRIEFQRHAIFYTVRDTDILIA
RILHQQMNHKRHLL
Download Length: 285 bp
>T116343 NZ_CP034659:368088-368372 [Escherichia coli]
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA
GTGTATAAACTCACTGAACGGGCAGCAGACGACTTCGCCGGGATTTATGACTATACGCTCTTGAAATTTGGTGAAGCTCA
GGCAGACCATTATACAGACGCACTTGAGGCATTTTTCGAGACACTGGCCGGAATGCCAGATATGGGACGTGACTACCATA
CCGTTCCTGGTGTGATGCGCATTGAATTTCAGCGACATGCCATTTTTTATACGGTTCGTGATACGGATATTCTGATTGCG
CGTATTTTGCACCAACAGATGAACCATAAACGCCATTTACTGTAA
Antitoxin
Download Length: 81 a.a. Molecular weight: 8875.01 Da Isoelectric Point: 7.0130
>AT116343 WP_000124732.1 NZ_CP034659:367853-368095 [Escherichia coli]
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV
MARTTSVTIGEQLDSFISRLIDSGRYGSASEVMRSALRLLEQQETNDEVIRQAVIAGLESGESSLSLRDIAAQRKLRHRV
Download Length: 243 bp
>AT116343 NZ_CP034659:367853-368095 [Escherichia coli]
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA
ATGGCCAGAACAACAAGCGTTACGATTGGCGAACAACTCGATTCGTTTATTAGTCGATTAATAGACAGTGGCCGTTACGG
CTCTGCGAGTGAAGTTATGCGTTCTGCGCTGCGATTGCTGGAGCAACAGGAAACAAACGATGAAGTTATTCGTCAGGCTG
TTATTGCTGGGCTGGAAAGCGGTGAAAGCTCGCTATCACTGCGGGATATTGCAGCACAAAGAAAACTGAGACATCGTGTA
TAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A837F4A9 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A6P1V8C3 |