Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 87449..88092 | Replicon | plasmid unnamed |
| Accession | NZ_CP034659 | ||
| Organism | Escherichia coli strain ATCC 98082 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | - |
| Locus tag | EKH78_RS22680 | Protein ID | WP_088765932.1 |
| Coordinates | 87449..87865 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | EKH78_RS22685 | Protein ID | WP_001261282.1 |
| Coordinates | 87862..88092 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| EKH78_RS24820 | 82585..82671 | + | 87 | Protein_91 | hypothetical protein | - |
| EKH78_RS22645 (EKH78_22845) | 82668..83321 | + | 654 | Protein_92 | IS30-like element IS30 family transposase | - |
| EKH78_RS22650 (EKH78_22850) | 83363..84898 | - | 1536 | WP_001000409.1 | IS66 family transposase | - |
| EKH78_RS22655 (EKH78_22855) | 84948..85241 | - | 294 | WP_050542499.1 | IS66 family insertion sequence element accessory protein TnpB | - |
| EKH78_RS22660 (EKH78_22860) | 85310..86014 | + | 705 | WP_009364894.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS22665 (EKH78_22870) | 86168..86344 | + | 177 | Protein_96 | DUF2254 domain-containing protein | - |
| EKH78_RS22670 (EKH78_22875) | 86408..87112 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| EKH78_RS22675 (EKH78_22880) | 87160..87351 | + | 192 | Protein_98 | helix-turn-helix domain-containing protein | - |
| EKH78_RS22680 (EKH78_22885) | 87449..87865 | - | 417 | WP_088765932.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| EKH78_RS22685 (EKH78_22890) | 87862..88092 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| EKH78_RS22690 (EKH78_22895) | 88049..88510 | + | 462 | WP_162535865.1 | hypothetical protein | - |
| EKH78_RS22695 (EKH78_22900) | 88654..89004 | + | 351 | WP_000493399.1 | hypothetical protein | - |
| EKH78_RS25000 | 89048..89170 | + | 123 | WP_257606738.1 | hypothetical protein | - |
| EKH78_RS22700 (EKH78_22905) | 89164..89961 | - | 798 | WP_000544830.1 | IS21-like element IS21 family helper ATPase IstB | - |
| EKH78_RS22705 (EKH78_22910) | 89961..91133 | - | 1173 | WP_042111630.1 | IS21-like element IS21 family transposase | - |
| EKH78_RS22710 (EKH78_22915) | 91219..91398 | + | 180 | Protein_106 | hypothetical protein | - |
| EKH78_RS22715 (EKH78_22925) | 91450..92161 | - | 712 | Protein_107 | IS6-like element IS26 family transposase | - |
| EKH78_RS22720 (EKH78_22935) | 92230..92670 | + | 441 | Protein_108 | ISNCY family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | mrkA / mrkB / mrkC / mrkD / mrkF | 1..454520 | 454520 | |
| - | inside | IScluster/Tn | - | - | 74292..117261 | 42969 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15164.56 Da Isoelectric Point: 6.4581
>T116342 WP_088765932.1 NZ_CP034659:c87865-87449 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
Download Length: 417 bp
>T116342 NZ_CP034659:c87865-87449 [Escherichia coli]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAGTTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT116342 WP_001261282.1 NZ_CP034659:c88092-87862 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT116342 NZ_CP034659:c88092-87862 [Escherichia coli]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|