Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4323276..4323773 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag EKH78_RS20765 Protein ID WP_001173343.1
Coordinates 4323600..4323773 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag EKH78_RS20760 Protein ID WP_000398619.1
Coordinates 4323276..4323572 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS20740 (4320007) 4320007..4320726 - 720 WP_000611288.1 two-component system response regulator DcuR -
EKH78_RS20745 (4320723) 4320723..4322354 - 1632 WP_001216477.1 sensor histidine kinase -
EKH78_RS20750 (4322535) 4322535..4322765 + 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
EKH78_RS20755 (4322777) 4322777..4323049 + 273 WP_000405651.1 GNAT family N-acetyltransferase -
EKH78_RS20760 (4323276) 4323276..4323572 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
EKH78_RS20765 (4323600) 4323600..4323773 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
EKH78_RS20770 (4323892) 4323892..4325409 - 1518 WP_001295074.1 lysine--tRNA ligase -
EKH78_RS20775 (4325646) 4325646..4327103 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T116334 WP_001173343.1 NZ_CP034658:4323600-4323773 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T116334 NZ_CP034658:4323600-4323773 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT116334 WP_000398619.1 NZ_CP034658:4323276-4323572 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT116334 NZ_CP034658:4323276-4323572 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References