Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yfjZ-ypjF/CbtA-CbeA
Location 2746362..2747029 Replicon chromosome
Accession NZ_CP034658
Organism Escherichia coli strain ATCC 98082

Toxin (Protein)


Gene name ypjF Uniprot ID Q46953
Locus tag EKH78_RS13350 Protein ID WP_001094400.1
Coordinates 2746700..2747029 (+) Length 110 a.a.

Antitoxin (Protein)


Gene name yfjZ Uniprot ID P52141
Locus tag EKH78_RS13345 Protein ID WP_000072690.1
Coordinates 2746362..2746679 (+) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKH78_RS13315 (2741414) 2741414..2742423 - 1010 Protein_2633 arsenic transporter -
EKH78_RS13320 (2742565) 2742565..2744268 + 1704 WP_000896263.1 protein YfjW -
EKH78_RS13325 (2744879) 2744879..2745063 + 185 Protein_2635 DUF905 family protein -
EKH78_RS24935 (2745031) 2745031..2745153 - 123 WP_001407482.1 hypothetical protein -
EKH78_RS13330 (2745166) 2745166..2745624 + 459 WP_000211841.1 antirestriction protein -
EKH78_RS13335 (2745633) 2745633..2746115 + 483 WP_001407480.1 RadC family protein -
EKH78_RS13340 (2746124) 2746124..2746324 + 201 WP_001395452.1 DUF987 domain-containing protein -
EKH78_RS13345 (2746362) 2746362..2746679 + 318 WP_000072690.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
EKH78_RS13350 (2746700) 2746700..2747029 + 330 WP_001094400.1 type IV toxin-antitoxin system toxin YpjF Toxin
EKH78_RS13355 (2747393) 2747393..2751973 - 4581 WP_010723187.1 adhesin-like autotransporter YpjA/EhaD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-108)

Antitoxin

Antitoxin CbeA

(5-104)


Sequences

Toxin        


Download         Length: 110 a.a.        Molecular weight: 12308.21 Da        Isoelectric Point: 7.2761

>T116326 WP_001094400.1 NZ_CP034658:2746700-2747029 [Escherichia coli]
MNTLPATISQAAKPCLSPVAVWQMLLTRLLEQHYGLTLNDTPFSDETVIKEHIDAGITLADAVNFLVEKYELVRIDHRGF
SWQQQSPYISVVDILRARRSTGLLKTNVK

Download         Length: 330 bp

>T116326 NZ_CP034658:2746700-2747029 [Escherichia coli]
ATGAACACTCTACCTGCTACAATTTCGCAGGCGGCGAAGCCCTGCCTGTCGCCAGTGGCTGTCTGGCAAATGCTACTGAC
ACGCCTGCTGGAACAACACTATGGCCTGACACTGAACGACACGCCGTTCAGTGATGAAACTGTTATTAAGGAACATATCG
ATGCTGGTATCACTCTGGCCGATGCAGTCAATTTTCTGGTGGAAAAGTACGAACTGGTACGTATCGATCACAGAGGATTT
TCGTGGCAACAACAGTCTCCATATATTTCCGTAGTAGATATTCTGCGAGCAAGGCGCTCTACCGGCTTGCTAAAAACTAA
TGTGAAATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11737.27 Da        Isoelectric Point: 6.4630

>AT116326 WP_000072690.1 NZ_CP034658:2746362-2746679 [Escherichia coli]
MSNTTWGLQRDITPRLGARLVQEGNQLHYLADRASITGKFSDAECPKLDVVFPHFISQIESMLTTGELNPRHAQCVTLYH
NGFTCEADTLGSCGYVYIAVYPTQR

Download         Length: 318 bp

>AT116326 NZ_CP034658:2746362-2746679 [Escherichia coli]
ATGAGCAACACCACATGGGGCCTGCAGCGAGATATCACGCCGCGCCTGGGAGCACGTCTGGTGCAGGAGGGCAACCAGCT
GCACTATCTGGCTGACCGGGCCAGTATCACCGGTAAGTTTAGTGACGCCGAATGTCCTAAGCTGGATGTGGTATTTCCAC
ATTTTATCAGCCAGATAGAGTCGATGCTGACCACTGGTGAACTGAATCCCCGCCATGCCCAATGCGTCACCCTGTACCAC
AACGGTTTTACCTGCGAAGCCGATACTCTTGGTAGTTGCGGCTACGTATACATCGCTGTTTACCCCACTCAACGCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A373F4I3

Antitoxin

Source ID Structure
PDB 2EA9
PDB 2JN7
AlphaFold DB P52141

References