Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3315713..3316331 Replicon chromosome
Accession NZ_CP034595
Organism Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag DK245_RS16415 Protein ID WP_001291435.1
Coordinates 3316113..3316331 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag DK245_RS16410 Protein ID WP_000344800.1
Coordinates 3315713..3316087 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK245_RS16400 3310802..3311995 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
DK245_RS16405 3312018..3315167 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
DK245_RS16410 3315713..3316087 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
DK245_RS16415 3316113..3316331 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
DK245_RS16420 3316503..3317054 + 552 WP_000102564.1 maltose O-acetyltransferase -
DK245_RS16425 3317170..3317640 + 471 WP_000136192.1 YlaC family protein -
DK245_RS16430 3317804..3319354 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
DK245_RS16435 3319396..3319749 - 354 WP_000878140.1 DUF1428 domain-containing protein -
DK245_RS16445 3320128..3320439 + 312 WP_000409911.1 MGMT family protein -
DK245_RS16450 3320470..3321042 - 573 WP_000779842.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T116278 WP_001291435.1 NZ_CP034595:3316113-3316331 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T116278 NZ_CP034595:3316113-3316331 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT116278 WP_000344800.1 NZ_CP034595:3315713-3316087 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT116278 NZ_CP034595:3315713-3316087 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References