Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1666598..1667429 Replicon chromosome
Accession NZ_CP034595
Organism Escherichia coli strain WCHEC035053S1G0

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag DK245_RS08040 Protein ID WP_000854814.1
Coordinates 1666598..1666972 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID P76364
Locus tag DK245_RS08045 Protein ID WP_001285584.1
Coordinates 1667061..1667429 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
DK245_RS08000 1661994..1663160 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
DK245_RS08005 1663279..1663752 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
DK245_RS08010 1663950..1665008 + 1059 WP_001200891.1 FUSC family protein -
DK245_RS08015 1665180..1665509 + 330 WP_000450409.1 DUF496 family protein -
DK245_RS08020 1665610..1665792 - 183 WP_001016348.1 hypothetical protein -
DK245_RS08025 1665864..1665992 + 129 Protein_1557 transposase domain-containing protein -
DK245_RS08030 1666281..1666394 - 114 WP_001161660.1 DUF957 domain-containing protein -
DK245_RS08035 1666407..1666601 - 195 WP_000988600.1 hypothetical protein -
DK245_RS08040 1666598..1666972 - 375 WP_000854814.1 type IV toxin-antitoxin system toxin CbtA Toxin
DK245_RS08045 1667061..1667429 - 369 WP_001285584.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
DK245_RS08050 1667503..1667724 - 222 WP_000692323.1 DUF987 domain-containing protein -
DK245_RS08055 1667787..1668233 - 447 WP_000187523.1 RadC family protein -
DK245_RS08060 1668230..1669762 - 1533 WP_001350525.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T116266 WP_000854814.1 NZ_CP034595:c1666972-1666598 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T116266 NZ_CP034595:c1666972-1666598 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13651.52 Da        Isoelectric Point: 5.9541

>AT116266 WP_001285584.1 NZ_CP034595:c1667429-1667061 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT116266 NZ_CP034595:c1667429-1667061 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
PDB 2H28
AlphaFold DB A0A1M2E8G6

References