Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 39153..39754 Replicon plasmid pL37-2
Accession NZ_CP034590
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag EKM58_RS22350 Protein ID WP_001216034.1
Coordinates 39153..39533 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag EKM58_RS22355 Protein ID WP_001190712.1
Coordinates 39533..39754 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS22310 35275..35973 - 699 Protein_32 IS1 family transposase -
EKM58_RS22320 36144..36353 + 210 WP_000874156.1 hypothetical protein -
EKM58_RS22325 36464..37315 + 852 WP_000611664.1 hypothetical protein -
EKM58_RS22330 37348..37710 - 363 Protein_35 phage antirepressor KilAC domain-containing protein -
EKM58_RS22335 37766..38463 + 698 Protein_36 IS1 family transposase -
EKM58_RS22340 38477..38941 - 465 Protein_37 hypothetical protein -
EKM58_RS22345 38969..39148 - 180 WP_038997082.1 hypothetical protein -
EKM58_RS22350 39153..39533 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
EKM58_RS22355 39533..39754 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EKM58_RS22360 39937..41493 + 1557 WP_038997084.1 type I restriction-modification system subunit M -
EKM58_RS22365 41490..42728 + 1239 WP_038997080.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / aac(3)-IId / floR / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..145704 145704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T116251 WP_001216034.1 NZ_CP034590:c39533-39153 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T116251 NZ_CP034590:c39533-39153 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGCATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT116251 WP_001190712.1 NZ_CP034590:c39754-39533 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT116251 NZ_CP034590:c39754-39533 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References