Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 8012..8547 Replicon plasmid pL37-2
Accession NZ_CP034590
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name relE Uniprot ID A0A0K4CX11
Locus tag EKM58_RS22170 Protein ID WP_000222771.1
Coordinates 8012..8299 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID T1PXV2
Locus tag EKM58_RS22175 Protein ID WP_001139206.1
Coordinates 8296..8547 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS22155 3637..7152 + 3516 WP_072664206.1 autotransporter outer membrane beta-barrel domain-containing protein -
EKM58_RS22160 7327..7532 - 206 Protein_4 transposase -
EKM58_RS22165 7522..7722 + 201 WP_000896262.1 DUF839 domain-containing protein -
EKM58_RS22170 8012..8299 - 288 WP_000222771.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EKM58_RS22175 8296..8547 - 252 WP_001139206.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
EKM58_RS22185 9160..13044 + 3885 WP_114213872.1 helicase -
EKM58_RS22190 13078..13518 + 441 WP_023352064.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid tet(A) / qnrS1 / aac(3)-IId / floR / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..145704 145704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11116.17 Da        Isoelectric Point: 10.5833

>T116250 WP_000222771.1 NZ_CP034590:c8299-8012 [Escherichia coli]
MTYTVKFRDDALKEWLRLDKTIQQQFAKKLKKCSENPHIASAKLRGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGK
RERSNVYHLASERMK

Download         Length: 288 bp

>T116250 NZ_CP034590:c8299-8012 [Escherichia coli]
ATGACTTATACAGTAAAATTCCGGGATGATGCACTGAAAGAATGGCTCAGACTGGACAAGACCATTCAGCAGCAGTTCGC
AAAAAAACTGAAAAAATGCAGTGAAAATCCTCATATAGCTTCTGCAAAACTACGTGGATTAAAGGACTGCTATAAAATCA
AACTTCGCGCATCAGGTTTTCGTCTGGTTTATCAGGTTATTGATGATATGTTAATTATTGCGGTCGTCGCAGTAGGTAAA
CGTGAACGCAGCAACGTTTATCATCTCGCCAGTGAACGGATGAAATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9171.39 Da        Isoelectric Point: 4.0927

>AT116250 WP_001139206.1 NZ_CP034590:c8547-8296 [Escherichia coli]
MPSIILSDTSASVSELKKNPMATVSAGEGYPVAILNRNQPAFYCVPAELYERMLDALDDQELVKLVTERSSQPLHDVDLD
SYL

Download         Length: 252 bp

>AT116250 NZ_CP034590:c8547-8296 [Escherichia coli]
ATGCCCAGTATCATTTTGTCTGACACCAGTGCAAGCGTCAGTGAGCTGAAAAAAAATCCGATGGCAACTGTCAGTGCCGG
AGAGGGGTATCCTGTTGCCATTCTTAACCGGAATCAGCCAGCATTCTACTGTGTCCCTGCTGAGCTCTATGAAAGAATGC
TTGATGCCTTGGATGACCAGGAGCTGGTAAAACTGGTTACTGAACGCAGCAGTCAGCCTCTGCATGATGTGGACCTGGAC
AGTTATTTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0K4CX11

Antitoxin

Source ID Structure
AlphaFold DB T1PXV2

References