Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3854490..3855144 Replicon chromosome
Accession NZ_CP034589
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag EKM58_RS18730 Protein ID WP_000244777.1
Coordinates 3854490..3854897 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag EKM58_RS18735 Protein ID WP_000354046.1
Coordinates 3854878..3855144 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS18710 3850447..3852180 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
EKM58_RS18715 3852186..3852896 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
EKM58_RS18720 3852921..3853817 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
EKM58_RS18725 3853929..3854450 + 522 WP_001055874.1 flavodoxin FldB -
EKM58_RS18730 3854490..3854897 - 408 WP_000244777.1 toxin CptA Toxin
EKM58_RS18735 3854878..3855144 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
EKM58_RS18740 3855387..3856367 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
EKM58_RS18745 3856563..3857222 - 660 WP_000250274.1 hemolysin III family protein -
EKM58_RS18750 3857386..3857697 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
EKM58_RS18755 3857742..3859175 + 1434 WP_063502079.1 6-phospho-beta-glucosidase BglA -
EKM58_RS18760 3859232..3859975 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T116247 WP_000244777.1 NZ_CP034589:c3854897-3854490 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T116247 NZ_CP034589:c3854897-3854490 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT116247 WP_000354046.1 NZ_CP034589:c3855144-3854878 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT116247 NZ_CP034589:c3855144-3854878 [Escherichia coli]
ATGGATATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References