Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2250202..2250840 Replicon chromosome
Accession NZ_CP034589
Organism Escherichia coli strain L37

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag EKM58_RS10850 Protein ID WP_000813794.1
Coordinates 2250202..2250378 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag EKM58_RS10855 Protein ID WP_001270286.1
Coordinates 2250424..2250840 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKM58_RS10830 2245821..2247035 - 1215 WP_071597387.1 BenE family transporter YdcO -
EKM58_RS10835 2247088..2247624 + 537 WP_000429133.1 DNA-binding transcriptional regulator SutR -
EKM58_RS10840 2247697..2249658 + 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
EKM58_RS10845 2249750..2249980 - 231 WP_000494244.1 YncJ family protein -
EKM58_RS10850 2250202..2250378 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
EKM58_RS10855 2250424..2250840 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
EKM58_RS10860 2250919..2252325 + 1407 WP_000760585.1 PLP-dependent aminotransferase family protein -
EKM58_RS10865 2252570..2253715 + 1146 WP_000047424.1 ABC transporter substrate-binding protein -
EKM58_RS10870 2253733..2254746 + 1014 WP_000220399.1 ABC transporter ATP-binding protein -
EKM58_RS10875 2254747..2255688 + 942 WP_001251313.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2244516..2245781 1265


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T116235 WP_000813794.1 NZ_CP034589:2250202-2250378 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T116235 NZ_CP034589:2250202-2250378 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT116235 WP_001270286.1 NZ_CP034589:2250424-2250840 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT116235 NZ_CP034589:2250424-2250840 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References