Detailed information of TA system TA116229

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1367924..1368843
• Replicon: chromosome
• Accession: NZ_CP034569
• Organism: Bacillus licheniformis strain ATCC 14580

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: T5HIF3
• Locus tag: EJ992_RS06910
• Protein ID: WP_003180879.1
• Coordinates: 1368097..1368843 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: Q65L00
• Locus tag: EJ992_RS06905
• Protein ID: WP_011197812.1
• Coordinates: 1367924..1368097 (-)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ992_RS06885	1363349..1364446	+	1098	WP_003180867.1	mannonate dehydratase	-
EJ992_RS06890	1364422..1365270	+	849	WP_009328728.1	SDR family oxidoreductase	-
EJ992_RS06895	1365297..1366310	+	1014	WP_011197810.1	zinc-binding alcohol dehydrogenase family protein	-
EJ992_RS06900	1366363..1367634	+	1272	WP_011197811.1	MFS transporter	-
EJ992_RS06905	1367924..1368097	-	174	WP_011197812.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
EJ992_RS06910	1368097..1368843	-	747	WP_003180879.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EJ992_RS06915	1368954..1369952	-	999	WP_009328723.1	inorganic phosphate transporter	-
EJ992_RS06920	1369965..1370582	-	618	WP_003180884.1	DUF47 domain-containing protein	-
EJ992_RS06925	1370914..1372671	+	1758	WP_011197813.1	gamma-glutamyltransferase	-
EJ992_RS06930	1372800..1373444	+	645	WP_009328721.1	YesL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-57)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29391.80 Da
• Isoelectric Point: 4.5367

>T116229 WP_003180879.1 NZ_CP034569:c1368843-1368097 [Bacillus licheniformis]
MLLFYQFLVWLIVLALALYVAAVWRFEKQLAEKTVAIRKTWYLLYVIGAVIYWTHDPQSIFTNPLHYLIVAVFFTLTDAF
IFLNAYFKKLGSSELATDTRMLLEENNDLLHTYQNRLKTFQYLLKNEPIHIYYGNIEAYAEGIEKLIKRFAEKMNISAAL
CEYNSEESKDHLLEHMENRFDVQEKLDRKDVYYEENGKMVLIPFSIHDFDYVMKLTSEDLVTEFDYLLFTSLTSIYDLLL
PNEEEGDD

Nucleotide

• Length: 747 bp

>T116229 NZ_CP034569:c1368843-1368097 [Bacillus licheniformis]
ATGCTGCTATTTTATCAATTCCTTGTTTGGCTTATTGTTTTGGCGCTGGCTCTGTACGTAGCTGCCGTGTGGCGTTTTGA
AAAACAGCTCGCCGAAAAAACGGTCGCCATCAGAAAAACGTGGTACCTTCTTTATGTCATCGGCGCCGTGATATATTGGA
CGCACGATCCGCAGTCAATTTTCACAAACCCGCTTCATTACCTGATCGTTGCCGTTTTTTTCACGTTGACAGACGCTTTT
ATTTTCTTAAATGCCTACTTTAAAAAGCTCGGCAGCTCTGAACTCGCGACAGATACAAGAATGCTTCTCGAAGAAAACAA
CGACCTCCTCCACACGTATCAAAACAGGCTGAAAACGTTTCAATACCTATTGAAAAACGAACCGATCCACATCTATTATG
GAAATATAGAAGCGTATGCAGAAGGCATCGAAAAGCTCATCAAACGGTTTGCCGAAAAAATGAATATATCCGCTGCACTT
TGCGAATATAATTCAGAAGAAAGCAAGGATCATTTGCTTGAGCATATGGAAAACCGGTTCGATGTTCAAGAAAAGCTTGA
CCGCAAAGACGTTTATTATGAAGAAAACGGAAAAATGGTCCTCATTCCTTTTTCCATCCACGATTTTGACTATGTCATGA
AGTTAACCTCAGAAGACCTTGTCACAGAATTTGATTATCTCCTGTTTACTTCTTTAACAAGCATCTATGATCTTCTGCTA
CCAAACGAAGAGGAAGGTGACGACTGA

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6971.08 Da
• Isoelectric Point: 10.7815

>AT116229 WP_011197812.1 NZ_CP034569:c1368097-1367924 [Bacillus licheniformis]
MEPAFQQDQESVKKRLAFKMFKRQSKTNIAKYEVSPYTERIFRQNERLIGEYKRKKA

Nucleotide

• Length: 174 bp

>AT116229 NZ_CP034569:c1368097-1367924 [Bacillus licheniformis]
ATGGAGCCTGCTTTTCAGCAAGACCAGGAAAGCGTAAAAAAGCGCCTCGCCTTTAAAATGTTTAAAAGGCAATCCAAAAC
CAATATCGCGAAATACGAAGTCAGCCCCTATACCGAACGGATTTTCCGGCAAAACGAACGGCTGATCGGCGAGTATAAAA
GAAAAAAAGCTTAA

Structures

Toxin

• AlphaFold DB: T5HIF3

Antitoxin

• AlphaFold DB: Q65L00