Detailed information of TA system TA116227

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-RHH
• Location: 12314..12852
• Replicon: plasmid p2
• Accession: NZ_CP034568
• Organism: Vibrio parahaemolyticus strain D3112

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: EKH72_RS25880
• Protein ID: WP_029865329.1
• Coordinates: 12314..12613 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: EKH72_RS25885
• Protein ID: WP_029865327.1
• Coordinates: 12610..12852 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EKH72_RS25880	12314..12613	-	300	WP_029865329.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EKH72_RS25885	12610..12852	-	243	WP_029865327.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
EKH72_RS25890	13128..14564	+	1437	WP_129831088.1	hypothetical protein	-
EKH72_RS25895	15194..15442	+	249	WP_023623173.1	CRISPR-associated endonuclease Cas2	-
EKH72_RS25900	15950..16360	+	411	WP_129831089.1	hypothetical protein	-
EKH72_RS25905	16411..16854	+	444	WP_129831090.1	hypothetical protein	-
EKH72_RS25910	16860..17444	-	585	WP_129831091.1	DUF2913 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94438	94438

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-92)

Antitoxin

• Antitoxin ParD (1-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11602.27 Da
• Isoelectric Point: 9.9225

>T116227 WP_029865329.1 NZ_CP034568:c12613-12314 [Vibrio parahaemolyticus]
VKPFQLTNKAKSDLRDIALFTSRRWGREQRNIYLKQFDDSFWLLAENPDIGKACDEVRDGYRKFPQGSHVIFYRQIGSQS
IEIIRILHKSMDVNPIFGA

Nucleotide

• Length: 300 bp

>T116227 NZ_CP034568:c12613-12314 [Vibrio parahaemolyticus]
GTGAAACCATTTCAATTAACTAACAAGGCGAAATCTGATTTAAGAGATATAGCCTTGTTTACCTCTCGACGCTGGGGTCG
TGAACAGCGAAACATTTACCTAAAACAGTTTGATGACTCATTTTGGCTATTAGCCGAAAATCCTGATATTGGTAAAGCTT
GTGACGAAGTTCGTGATGGATACAGAAAATTCCCACAAGGAAGCCACGTAATATTTTATCGACAGATCGGCAGTCAAAGT
ATTGAGATTATCCGTATACTCCACAAAAGCATGGATGTGAACCCAATTTTTGGCGCATAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8983.90 Da
• Isoelectric Point: 4.2213

>AT116227 WP_029865327.1 NZ_CP034568:c12852-12610 [Vibrio parahaemolyticus]
MAKNTSITLGDHFDGFITNQIQSGRYGSASEVIRSALRLLETQETKMNTLRQLLVEGEESGVADYDLDSFINELDSEERK

Nucleotide

• Length: 243 bp

>AT116227 NZ_CP034568:c12852-12610 [Vibrio parahaemolyticus]
ATGGCTAAGAACACTAGTATTACTTTGGGTGACCATTTTGACGGTTTCATTACTAATCAAATCCAAAGTGGTCGCTATGG
ATCAGCAAGTGAAGTTATTCGTTCTGCGTTACGTTTACTTGAAACCCAAGAAACTAAAATGAACACTTTACGCCAATTGC
TGGTTGAAGGTGAAGAGAGTGGCGTTGCGGATTATGACCTTGATTCGTTTATCAATGAGTTAGATAGCGAAGAAAGAAAG
TGA