Detailed information of TA system TA116222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 1665759..1666350
• Replicon: chromosome
• Accession: NZ_CP034562
• Organism: Flammeovirga pectinis strain L12M1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: EI427_RS06670
• Protein ID: WP_126612950.1
• Coordinates: 1665759..1666055 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: EI427_RS06675
• Protein ID: WP_126612952.1
• Coordinates: 1666063..1666350 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EI427_RS06650	1661902..1662240	+	339	WP_126612942.1	tetratricopeptide repeat protein	-
EI427_RS06655	1662221..1663609	+	1389	WP_126612944.1	exodeoxyribonuclease VII large subunit	-
EI427_RS06660	1663652..1663855	+	204	WP_126612946.1	exodeoxyribonuclease VII small subunit	-
EI427_RS06665	1663996..1665159	+	1164	WP_170178413.1	ABC transporter permease	-
EI427_RS06670	1665759..1666055	+	297	WP_126612950.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EI427_RS06675	1666063..1666350	+	288	WP_126612952.1	putative addiction module antidote protein	Antitoxin
EI427_RS06680	1666758..1669169	-	2412	WP_126612954.1	alpha-ketoacid dehydrogenase subunit alpha/beta	-
EI427_RS06685	1669347..1670825	-	1479	WP_126612956.1	transporter substrate-binding domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11578.67 Da
• Isoelectric Point: 10.4165

>T116222 WP_126612950.1 NZ_CP034562:1665759-1666055 [Flammeovirga pectinis]
MYKLEKTDIFDKWLRKLKDRRAKVKILFRLQLIEEKGHFGDCEPIGKGLSELRIHYGKGYRIYLKKKNDKIVILLSGGDK
STQNKDIDKAIDIWQSLK

Nucleotide

• Length: 297 bp

>T116222 NZ_CP034562:1665759-1666055 [Flammeovirga pectinis]
ATGTATAAGTTAGAAAAGACGGACATTTTTGATAAATGGTTAAGAAAGTTAAAAGATCGACGAGCAAAAGTGAAAATCTT
ATTTAGGTTACAATTAATAGAAGAGAAGGGTCATTTTGGAGACTGTGAGCCAATCGGTAAAGGTTTAAGTGAGTTGAGAA
TTCATTATGGGAAAGGGTATCGAATTTACCTTAAGAAGAAGAATGATAAGATTGTTATACTTTTATCTGGAGGTGATAAA
TCAACCCAAAATAAAGATATTGATAAGGCTATTGATATTTGGCAATCATTAAAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10358.75 Da
• Isoelectric Point: 4.4791

>AT116222 WP_126612952.1 NZ_CP034562:1666063-1666350 [Flammeovirga pectinis]
MKEEVSKLNISEYLDDDDMIAEYLNMVLEEGDSNDIITALGHISKAIGMSKIAEQTGLSRTSLYKALSEGSKPQFETILK
VVRAIGGNLHVNTAS

Nucleotide

• Length: 288 bp

>AT116222 NZ_CP034562:1666063-1666350 [Flammeovirga pectinis]
ATGAAAGAGGAAGTATCAAAATTAAATATATCAGAATACCTAGATGATGATGACATGATCGCTGAATATCTAAACATGGT
TTTAGAGGAGGGTGATTCAAATGATATCATTACAGCTTTAGGTCATATTTCAAAAGCTATTGGTATGTCAAAGATTGCAG
AACAGACTGGTTTAAGTAGAACAAGTCTTTATAAAGCACTTTCAGAAGGAAGTAAACCTCAATTTGAAACAATATTAAAG
GTTGTAAGAGCTATTGGTGGGAATTTACATGTAAATACTGCATCTTAA