Detailed information of TA system TA116218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 2574543..2575198
• Replicon: chromosome
• Accession: NZ_CP034554
• Organism: Burkholderia cepacia ATCC 25416

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2HGC0
• Locus tag: EJ998_RS30340
• Protein ID: WP_021158162.1
• Coordinates: 2574543..2574962 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HMP5
• Locus tag: EJ998_RS30345
• Protein ID: WP_021158161.1
• Coordinates: 2574959..2575198 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ998_RS30315	2569900..2570694	+	795	WP_027789489.1	AraC family transcriptional regulator	-
EJ998_RS30320	2570954..2571907	+	954	WP_027789490.1	DMT family transporter	-
EJ998_RS30325	2572092..2572640	+	549	WP_027789491.1	hypothetical protein	-
EJ998_RS30330	2572744..2572878	-	135	WP_021158165.1	entericidin A/B family lipoprotein	-
EJ998_RS30335	2573112..2574407	+	1296	WP_027789492.1	aspartate carbamoyltransferase	-
EJ998_RS30340	2574543..2574962	-	420	WP_021158162.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJ998_RS30345	2574959..2575198	-	240	WP_021158161.1	antitoxin VapB	Antitoxin
EJ998_RS30350	2575508..2577754	+	2247	WP_027789494.1	TonB-dependent siderophore receptor	-
EJ998_RS30355	2577758..2578513	+	756	WP_027789495.1	sel1 repeat family protein	-
EJ998_RS30360	2578510..2579193	+	684	WP_027789496.1	Fe2+-dependent dioxygenase	-
EJ998_RS30365	2579282..2579521	+	240	WP_011659184.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EJ998_RS30370	2579518..2579913	+	396	WP_027789497.1	type II toxin-antitoxin system VapC family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15212.57 Da
• Isoelectric Point: 5.9052

>T116218 WP_021158162.1 NZ_CP034554:c2574962-2574543 [Burkholderia cepacia ATCC 25416]
MILVDTNVISEPLRREPNAAVIEWLDAQNVETLFLAAISLAEIRLGVAVLPEGRRREWLHQSIEQRVLPLFRGRILPFDD
AASKAYASLRARARAAGVAIAPSDGFIAGTAEANGLIVATRDVTPFEAMGIRVIDPWAR

Nucleotide

• Length: 420 bp

>T116218 NZ_CP034554:c2574962-2574543 [Burkholderia cepacia ATCC 25416]
ATGATCCTTGTTGATACGAATGTCATTTCCGAACCGCTACGACGCGAGCCGAACGCGGCCGTGATCGAGTGGCTCGATGC
CCAGAATGTCGAAACCTTGTTTCTGGCTGCGATCAGTCTCGCGGAAATACGATTGGGCGTGGCTGTATTGCCGGAAGGAC
GCCGGCGAGAGTGGCTGCATCAGAGCATCGAGCAGCGCGTGCTGCCATTGTTTCGCGGACGAATCCTGCCATTCGACGAT
GCTGCGAGCAAAGCGTATGCGAGCCTTCGCGCACGCGCTCGTGCCGCAGGTGTCGCAATTGCACCATCCGATGGTTTTAT
CGCCGGCACGGCCGAAGCGAACGGCCTGATCGTCGCCACGCGCGATGTCACGCCGTTCGAGGCAATGGGAATTCGCGTAA
TCGACCCGTGGGCGCGCTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 8629.79 Da
• Isoelectric Point: 5.6643

>AT116218 WP_021158161.1 NZ_CP034554:c2575198-2574959 [Burkholderia cepacia ATCC 25416]
MPVITVRNLSDEVHRALRIRAAQHGRSTEAEVRDILEQAVLPGGRLKLGTMLAAIGQEAGGFDFDAQRDKTPADPMSFE

Nucleotide

• Length: 240 bp

>AT116218 NZ_CP034554:c2575198-2574959 [Burkholderia cepacia ATCC 25416]
ATGCCGGTGATCACCGTCCGAAATCTGTCCGATGAAGTGCATCGAGCACTTCGCATTCGCGCGGCCCAACATGGGCGCAG
CACCGAAGCCGAGGTGCGCGACATTCTCGAACAGGCCGTTCTTCCGGGCGGGCGGCTCAAGCTGGGAACGATGCTGGCGG
CAATCGGGCAGGAGGCCGGCGGATTCGATTTCGACGCCCAGCGCGACAAGACGCCTGCCGATCCGATGAGCTTCGAATGA

Structures

Toxin

• AlphaFold DB: U2HGC0

Antitoxin

• AlphaFold DB: U2HMP5