116215

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PP_4151-4152/excise(antitoxin)
Location 1709520..1710559 Replicon chromosome
Accession NZ_CP034554
Organism Burkholderia cepacia ATCC 25416

Toxin (Protein)


Gene name PP_4152 Uniprot ID A0A806V135
Locus tag EJ998_RS26325 Protein ID WP_027791437.1
Coordinates 1709984..1710559 (+) Length 192 a.a.

Antitoxin (Protein)


Gene name PP_4151 Uniprot ID A0A806V2D4
Locus tag EJ998_RS26320 Protein ID WP_027791436.1
Coordinates 1709520..1709987 (+) Length 156 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJ998_RS26295 1704980..1706443 + 1464 WP_027791432.1 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase -
EJ998_RS26300 1706493..1707341 + 849 WP_027791433.1 3,4-dihydroxyphenylacetate 2,3-dioxygenase -
EJ998_RS26305 1707375..1707767 + 393 WP_027791434.1 5-carboxymethyl-2-hydroxymuconate Delta-isomerase -
EJ998_RS26310 1707788..1708591 + 804 WP_021164056.1 2-oxo-hepta-3-ene-1,7-dioic acid hydratase -
EJ998_RS26315 1708604..1709410 + 807 WP_027791435.1 4-hydroxy-2-oxoheptanedioate aldolase -
EJ998_RS26320 1709520..1709987 + 468 WP_027791436.1 helix-turn-helix domain-containing protein Antitoxin
EJ998_RS26325 1709984..1710559 + 576 WP_027791437.1 PIN domain-containing protein Toxin
EJ998_RS26330 1710615..1711394 - 780 WP_027791438.1 helix-turn-helix transcriptional regulator -
EJ998_RS26335 1711490..1711741 + 252 WP_027791439.1 hypothetical protein -
EJ998_RS26340 1711788..1712753 + 966 WP_027791440.1 zinc-binding alcohol dehydrogenase family protein -
EJ998_RS26345 1712924..1714015 - 1092 WP_034196044.1 porin -
EJ998_RS26350 1714175..1715242 - 1068 WP_027791442.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(9-115)

Antitoxin

Helix-turn-helix domain, group 17

(84-132)


Sequences

Toxin        


Download         Length: 192 a.a.        Molecular weight: 21502.82 Da        Isoelectric Point: 5.6556

>T116215 WP_027791437.1 NZ_CP034554:1709984-1710559 [Burkholderia cepacia ATCC 25416]
MTSSPFTAVYDACVLYPAPLRDFLMWLALSGCFRARWSVDIHDEWKRNLLRNRADLTAAQLDRTASLMDRAIPDALVTGY
EALIGGLTLPDANDRHVLAAAIRCNASVIVTFNQKDFPEEVLSIYGVEAQHPDVFVDNLFDLEPAAVVAAARRQRETLKH
PPMIVERYLEILQRLGMVQTCKALEAYRTII

Download         Length: 576 bp

>T116215 NZ_CP034554:1709984-1710559 [Burkholderia cepacia ATCC 25416]
ATGACGAGTTCGCCATTCACGGCGGTCTACGATGCGTGCGTGCTTTATCCCGCACCGCTGCGTGATTTCCTGATGTGGCT
GGCGCTGAGCGGATGTTTTCGGGCTCGCTGGAGTGTGGATATTCATGACGAATGGAAACGGAATCTGCTGCGTAACCGGG
CCGATCTGACCGCGGCGCAACTCGATCGCACGGCAAGCCTGATGGATAGGGCGATACCCGATGCGCTCGTTACCGGTTAC
GAGGCGCTGATTGGTGGGCTGACGCTGCCGGACGCGAACGATCGACATGTGCTCGCTGCCGCGATCCGCTGCAACGCCAG
CGTGATCGTTACGTTCAATCAGAAGGATTTCCCCGAAGAAGTGTTGTCGATCTACGGTGTCGAAGCACAGCACCCTGACG
TGTTCGTCGACAATCTCTTCGATCTCGAACCGGCCGCAGTGGTCGCCGCCGCTCGGCGGCAGCGCGAAACACTCAAGCAT
CCACCGATGATTGTGGAGCGCTATCTGGAAATCTTGCAGCGTCTGGGGATGGTTCAGACCTGCAAGGCACTCGAAGCCTA
TCGGACGATTATTTGA

Antitoxin


Download         Length: 156 a.a.        Molecular weight: 17497.87 Da        Isoelectric Point: 6.2956

>AT116215 WP_027791436.1 NZ_CP034554:1709520-1709987 [Burkholderia cepacia ATCC 25416]
MPHTTMSETILPVEREMRAAVEGQRALAAYLATRTEIQRIQIFDDENRAHQVELPTSALRLLVDILAELADGNAVKVVPV
HAELTTQEAADLLNVSRPHLIKLLESNALPHHKTGKHRRVRFSDLMRYKAERDQASADAMEELSRQAQEHGMGYE

Download         Length: 468 bp

>AT116215 NZ_CP034554:1709520-1709987 [Burkholderia cepacia ATCC 25416]
ATGCCTCACACCACGATGTCCGAAACGATCTTGCCTGTCGAGCGCGAGATGCGGGCGGCTGTCGAAGGGCAGCGTGCGCT
TGCGGCATACCTGGCAACGCGAACGGAAATACAGCGCATCCAGATCTTCGACGACGAAAATCGCGCGCATCAGGTCGAAC
TTCCGACCTCCGCGCTGCGTCTGCTGGTCGATATCCTCGCCGAGTTGGCCGACGGCAATGCGGTCAAGGTCGTGCCGGTT
CATGCGGAACTGACGACACAGGAAGCGGCCGACCTGTTGAACGTTTCTCGTCCGCACCTGATCAAGCTGCTCGAATCGAA
TGCGCTTCCGCACCACAAGACCGGCAAGCATCGCCGTGTGCGATTTTCCGACCTGATGCGTTACAAGGCGGAACGCGATC
AGGCAAGCGCGGACGCGATGGAGGAGTTGAGCCGGCAGGCGCAAGAGCACGGGATGGGGTACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A806V135

Antitoxin

Source ID Structure
AlphaFold DB A0A806V2D4

References