Detailed information of TA system TA116214

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1505618..1506195
• Replicon: chromosome
• Accession: NZ_CP034554
• Organism: Burkholderia cepacia ATCC 25416

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: U2G063
• Locus tag: EJ998_RS25465
• Protein ID: WP_021160897.1
• Coordinates: 1505618..1505983 (-)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U2HEK9
• Locus tag: EJ998_RS25470
• Protein ID: WP_021160898.1
• Coordinates: 1505977..1506195 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ998_RS25445	1501397..1501951	+	555	WP_027791286.1	carboxymuconolactone decarboxylase family protein	-
EJ998_RS40845	1502273..1502887	-	615	WP_155253146.1	hypothetical protein	-
EJ998_RS25455	1502992..1504590	-	1599	WP_027791288.1	alkyl hydroperoxide reductase subunit F	-
EJ998_RS25460	1504716..1505279	-	564	WP_006488685.1	peroxiredoxin	-
EJ998_RS25465	1505618..1505983	-	366	WP_021160897.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJ998_RS25470	1505977..1506195	-	219	WP_021160898.1	hypothetical protein	Antitoxin
EJ998_RS25475	1506286..1507182	-	897	WP_027791289.1	LysR family transcriptional regulator	-
EJ998_RS25480	1507360..1508526	+	1167	WP_027791290.1	branched-chain amino acid ABC transporter substrate-binding protein	-
EJ998_RS25485	1508581..1509105	+	525	WP_021160901.1	L-2-amino-thiazoline-4-carboxylic acid hydrolase	-
EJ998_RS25490	1509098..1510363	+	1266	WP_027791291.1	Zn-dependent hydrolase	-
EJ998_RS25495	1510451..1510933	-	483	WP_027791292.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13448.50 Da
• Isoelectric Point: 8.4389

>T116214 WP_021160897.1 NZ_CP034554:c1505983-1505618 [Burkholderia cepacia ATCC 25416]
MVKALFDTNILIDYLGGVESARAELGRYDYRAISTISWMEVLVGTSAQDEAPIRAWLSSFDVIALDSPVANRAVTIRKER
RIRLPDAIVWASAQVNGLLLVSRNTKDFPATEPGVRVPYKL

Nucleotide

• Length: 366 bp

>T116214 NZ_CP034554:c1505983-1505618 [Burkholderia cepacia ATCC 25416]
ATGGTAAAAGCTCTCTTCGATACCAACATCCTGATCGACTATCTGGGCGGCGTGGAATCCGCTCGTGCGGAACTGGGCCG
CTACGACTATCGGGCGATCAGCACGATCTCGTGGATGGAGGTCCTGGTCGGCACGTCCGCACAGGACGAGGCACCCATCC
GCGCGTGGCTGTCGTCGTTCGATGTCATCGCACTGGACAGCCCGGTTGCGAATCGTGCCGTGACAATCCGCAAGGAACGC
CGCATACGCCTTCCCGATGCAATCGTCTGGGCATCGGCACAAGTGAACGGTCTACTGCTCGTGTCGCGCAACACGAAGGA
CTTCCCTGCCACCGAACCCGGCGTTCGCGTGCCCTACAAGCTCTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8372.51 Da
• Isoelectric Point: 5.0604

>AT116214 WP_021160898.1 NZ_CP034554:c1506195-1505977 [Burkholderia cepacia ATCC 25416]
MSRILVDLSDGQLDELAVIVETQHRPRAAIIRDAIDAYIALHKHRLADDVFGLWKDRKVDGLTYQEELRSEW

Nucleotide

• Length: 219 bp

>AT116214 NZ_CP034554:c1506195-1505977 [Burkholderia cepacia ATCC 25416]
ATGAGCCGAATTCTGGTCGATCTGTCGGACGGCCAACTCGACGAGTTGGCCGTCATCGTCGAGACTCAACATCGTCCCCG
CGCCGCAATCATCCGCGACGCGATCGATGCCTATATTGCCTTGCACAAGCACCGGCTTGCCGACGACGTCTTCGGTCTCT
GGAAAGATCGCAAGGTCGACGGGCTCACCTATCAGGAAGAACTGCGCTCGGAATGGTAA

Structures

Toxin

• AlphaFold DB: U2G063

Antitoxin

• AlphaFold DB: U2HEK9