Detailed information of TA system TA116213

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 1115212..1115853
• Replicon: chromosome
• Accession: NZ_CP034554
• Organism: Burkholderia cepacia ATCC 25416

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: EJ998_RS23630
• Protein ID: WP_027790991.1
• Coordinates: 1115515..1115853 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: U2GAF3
• Locus tag: EJ998_RS23625
• Protein ID: WP_021157290.1
• Coordinates: 1115212..1115511 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ998_RS23600	1110326..1111597	+	1272	WP_027790987.1	Rieske 2Fe-2S domain-containing protein	-
EJ998_RS23605	1111615..1112100	+	486	WP_034196026.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-
EJ998_RS23610	1112121..1112447	+	327	WP_006479527.1	non-heme iron oxygenase ferredoxin subunit	-
EJ998_RS23615	1112437..1113657	+	1221	WP_027790989.1	FAD-dependent oxidoreductase	-
EJ998_RS23620	1113869..1115155	+	1287	WP_027790990.1	DUF445 domain-containing protein	-
EJ998_RS23625	1115212..1115511	-	300	WP_021157290.1	putative addiction module antidote protein	Antitoxin
EJ998_RS23630	1115515..1115853	-	339	WP_027790991.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJ998_RS23640	1116193..1117833	+	1641	WP_027790992.1	acetolactate synthase large subunit	-
EJ998_RS23645	1117863..1119296	+	1434	WP_027790993.1	aldehyde dehydrogenase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-109)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12591.43 Da
• Isoelectric Point: 9.9308

>T116213 WP_027790991.1 NZ_CP034554:c1115853-1115515 [Burkholderia cepacia ATCC 25416]
MVDIPQSMPYSAPTFSIRTTDVFDAWFAGLRDRVAKRRIQARIDRLSMGNPGDWKSAGSPVVEMRIDHGPGYRVYYVRRG
TIWVILLGGGDKSTQQADIRAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T116213 NZ_CP034554:c1115853-1115515 [Burkholderia cepacia ATCC 25416]
ATGGTTGACATACCGCAATCAATGCCCTACAGTGCGCCTACGTTCAGCATCCGGACCACCGACGTCTTCGACGCATGGTT
TGCCGGCCTGCGCGATCGCGTCGCGAAGCGCCGCATCCAGGCACGCATCGACCGCCTGTCGATGGGCAATCCGGGCGACT
GGAAATCCGCGGGCTCGCCGGTCGTCGAAATGCGGATCGACCACGGCCCCGGCTATCGCGTCTACTACGTCCGGCGCGGA
ACGATCTGGGTCATCCTGCTCGGCGGCGGCGATAAATCGACGCAACAGGCCGACATACGCGCCGCGCACGCGATGCTCGC
GCACCTCGACATGGAGTAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10633.01 Da
• Isoelectric Point: 6.3217

>AT116213 WP_021157290.1 NZ_CP034554:c1115511-1115212 [Burkholderia cepacia ATCC 25416]
MDKISTRPWDSADHLKTEDDMADYFEACLQEAGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAHA

Nucleotide

• Length: 300 bp

>AT116213 NZ_CP034554:c1115511-1115212 [Burkholderia cepacia ATCC 25416]
ATGGACAAGATCAGCACCCGGCCGTGGGATTCGGCCGACCATCTGAAGACCGAAGACGACATGGCCGACTACTTCGAAGC
GTGCCTGCAGGAAGCCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCACGCGCACGCGGCATGTCGC
AGGTCGCGCGCGACGCGGGCCTGTCGCGCGAAGGGCTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACC
ATCCTGAAGGTCATCAAGGCGCTCGGCCTGCAACTGCACGGCGCGAAAGCGCATGCATGA

Structures

Antitoxin

• AlphaFold DB: U2GAF3