Detailed information of TA system TA116209

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2379337..2380315
• Replicon: chromosome
• Accession: NZ_CP034551
• Organism: Bacillus cereus ATCC 14579

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: EJ379_RS12455
• Protein ID: WP_000624977.1
• Coordinates: 2379337..2380074 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: EJ379_RS12460
• Protein ID: WP_000237819.1
• Coordinates: 2380187..2380315 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ379_RS12430	2374950..2375357	+	408	WP_000063587.1	VOC family protein	-
EJ379_RS12435	2375381..2375761	+	381	WP_000497668.1	methyltransferase	-
EJ379_RS12440	2375919..2377604	-	1686	WP_002195513.1	alpha-keto acid decarboxylase family protein	-
EJ379_RS12445	2377712..2378194	+	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
EJ379_RS12450	2378361..2379098	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EJ379_RS12455	2379337..2380074	+	738	WP_000624977.1	stage II sporulation protein SA	Toxin
EJ379_RS12460	2380187..2380315	+	129	WP_000237819.1	hypothetical protein	Antitoxin
EJ379_RS12465	2380388..2380564	+	177	WP_000852629.1	stage II sporulation protein SB	-
EJ379_RS12470	2380584..2380973	-	390	WP_000713863.1	YxeA family protein	-
EJ379_RS12475	2381185..2382654	+	1470	WP_000287556.1	beta-Ala-His dipeptidase	-
EJ379_RS12480	2382900..2383709	+	810	WP_000664546.1	C39 family peptidase	-
EJ379_RS12485	2383735..2384337	+	603	WP_000517266.1	hypothetical protein	-
EJ379_RS12490	2384581..2384832	-	252	WP_000827560.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T116209 WP_000624977.1 NZ_CP034551:2379337-2380074 [Bacillus cereus ATCC 14579]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T116209 NZ_CP034551:2379337-2380074 [Bacillus cereus ATCC 14579]
ATGATCTCTAACATTCGAATTGGCTTATTTGTTTTAGCGATTGTTTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACGCCTAATATTTCAGAAATATGGGGTGCGAAATTTAGCGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGGGAAATATACACGACAATTATTCAAAATGTTAATCCAGCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGCGCGAAAATT
GTTGTTTTTGAAGCCGCGAAAGAGTTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATCGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT116209 WP_000237819.1 NZ_CP034551:2380187-2380315 [Bacillus cereus ATCC 14579]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT116209 NZ_CP034551:2380187-2380315 [Bacillus cereus ATCC 14579]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2