116208

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 239823..240465 Replicon chromosome
Accession NZ_CP034551
Organism Bacillus cereus ATCC 14579

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag EJ379_RS01375 Protein ID WP_000635963.1
Coordinates 240115..240465 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EJ379_RS01370 Protein ID WP_000004570.1
Coordinates 239823..240110 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJ379_RS01345 235140..236102 + 963 WP_000961166.1 UV DNA damage repair endonuclease UvsE -
EJ379_RS01350 236095..236667 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
EJ379_RS01355 236761..237120 + 360 WP_000583416.1 holo-ACP synthase -
EJ379_RS01360 237277..238227 + 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
EJ379_RS01365 238345..239514 + 1170 WP_000390615.1 alanine racemase -
EJ379_RS01370 239823..240110 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EJ379_RS01375 240115..240465 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EJ379_RS01380 240533..242701 + 2169 WP_000426242.1 RNA-binding transcriptional accessory protein -
EJ379_RS01385 242759..242875 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EJ379_RS01390 243071..243529 + 459 WP_000344231.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T116208 WP_000635963.1 NZ_CP034551:240115-240465 [Bacillus cereus ATCC 14579]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T116208 NZ_CP034551:240115-240465 [Bacillus cereus ATCC 14579]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTACGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAGCGCTTAACGGACAAAATCACTCACTTAGATGAAGTGATGATGATTCGTGTAGATGAAGCGCT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT116208 WP_000004570.1 NZ_CP034551:239823-240110 [Bacillus cereus ATCC 14579]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT116208 NZ_CP034551:239823-240110 [Bacillus cereus ATCC 14579]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTACATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCA
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References