116207

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1580848..1581412 Replicon chromosome
Accession NZ_CP034550
Organism Saccharothrix syringae strain NRRL B-16468

Toxin (Protein)


Gene name mazF Uniprot ID A0A5Q0GV09
Locus tag EKG83_RS07500 Protein ID WP_033427529.1
Coordinates 1581065..1581412 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A5Q0GTS5
Locus tag EKG83_RS07495 Protein ID WP_033427530.1
Coordinates 1580848..1581078 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EKG83_RS07470 1576124..1576639 - 516 WP_033427533.1 hypothetical protein -
EKG83_RS07475 1576762..1577451 + 690 WP_051764359.1 TetR/AcrR family transcriptional regulator C-terminal domain-containing protein -
EKG83_RS07480 1577428..1578405 - 978 WP_051764357.1 LCP family protein -
EKG83_RS07485 1578500..1578883 + 384 WP_051764354.1 hypothetical protein -
EKG83_RS07490 1579011..1580825 + 1815 WP_170191746.1 glycosyltransferase -
EKG83_RS07495 1580848..1581078 + 231 WP_033427530.1 ribbon-helix-helix domain-containing protein Antitoxin
EKG83_RS07500 1581065..1581412 + 348 WP_033427529.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
EKG83_RS07505 1581535..1581729 - 195 WP_033427528.1 ferredoxin -
EKG83_RS07510 1581726..1582925 - 1200 WP_033427527.1 cytochrome P450 -
EKG83_RS07515 1583111..1583980 + 870 WP_084715949.1 ABC transporter ATP-binding protein -
EKG83_RS07520 1583977..1584828 + 852 WP_033427526.1 ABC transporter permease -
EKG83_RS07525 1584845..1585084 + 240 WP_033427525.1 helix-turn-helix transcriptional regulator -
EKG83_RS07530 1585206..1585796 - 591 WP_033427524.1 TetR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12478.43 Da        Isoelectric Point: 11.2857

>T116207 WP_033427529.1 NZ_CP034550:1581065-1581412 [Saccharothrix syringae]
MRRGDIYWVDLEPVRGTEANKVRPAVVVSNDAANRAAGRGGRGVVTVVPITSNTTRVYPFQVLLPARDCGLDRDSKAQAE
QVRTVAAVRLRARIGGLPPAVLKQLDEALRIHLAL

Download         Length: 348 bp

>T116207 NZ_CP034550:1581065-1581412 [Saccharothrix syringae]
ATGCGGCGAGGTGACATCTACTGGGTGGACCTGGAGCCGGTGCGCGGCACCGAGGCCAACAAGGTCCGCCCGGCCGTGGT
CGTGAGCAACGACGCGGCGAACCGGGCCGCCGGGCGGGGCGGCCGGGGCGTGGTGACCGTCGTGCCGATCACCTCGAACA
CCACGCGGGTGTACCCGTTCCAGGTGCTCCTGCCCGCCAGGGACTGCGGCCTGGACAGGGACTCCAAGGCGCAGGCCGAG
CAGGTGCGCACGGTGGCCGCGGTCCGGCTCCGCGCCCGCATCGGCGGTCTGCCGCCCGCCGTGCTCAAGCAGTTGGACGA
GGCGCTGAGGATCCACCTCGCGCTCTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8536.38 Da        Isoelectric Point: 4.1589

>AT116207 WP_033427530.1 NZ_CP034550:1580848-1581078 [Saccharothrix syringae]
MKISVSLPEEDVEFVDRYLRQHRSASRSSVIHQAIALLREAGLENAYATAWEEWEAEGEADMWDVTASDGVVDAAR

Download         Length: 231 bp

>AT116207 NZ_CP034550:1580848-1581078 [Saccharothrix syringae]
ATGAAAATCAGTGTGAGCCTGCCCGAGGAAGACGTAGAGTTCGTTGATCGCTACCTGCGGCAACATCGCAGCGCCTCCAG
GTCCTCGGTGATCCACCAGGCGATCGCGCTGCTCCGCGAGGCCGGACTGGAGAACGCCTACGCCACCGCGTGGGAGGAGT
GGGAAGCCGAGGGGGAAGCCGACATGTGGGACGTGACCGCGTCAGACGGTGTCGTCGATGCGGCGAGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q0GV09

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q0GTS5

References