Detailed information of TA system TA116199

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 694656..695297
• Replicon: chromosome
• Accession: NZ_CP034547
• Organism: Burkholderia cenocepacia strain YG-3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: D5R55_RS26745
• Protein ID: WP_126367358.1
• Coordinates: 694656..694994 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A228HNU1
• Locus tag: D5R55_RS26750
• Protein ID: WP_050017389.1
• Coordinates: 694998..695297 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D5R55_RS26730	691179..692612	-	1434	WP_126367354.1	aldehyde dehydrogenase family protein	-
D5R55_RS26735	692641..694281	-	1641	WP_126367356.1	acetolactate synthase large subunit	-
D5R55_RS26745	694656..694994	+	339	WP_126367358.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D5R55_RS26750	694998..695297	+	300	WP_050017389.1	putative addiction module antidote protein	Antitoxin
D5R55_RS37980	695679..695956	+	278	Protein_593	type II toxin-antitoxin system RelE/ParE family toxin	-
D5R55_RS26760	695969..696271	+	303	WP_126367362.1	HigA family addiction module antidote protein	-
D5R55_RS26765	696305..697591	-	1287	WP_126367364.1	DUF445 domain-containing protein	-
D5R55_RS26770	697702..698922	-	1221	WP_126367366.1	FAD-dependent oxidoreductase	-
D5R55_RS26775	698912..699238	-	327	WP_126367368.1	non-heme iron oxygenase ferredoxin subunit	-
D5R55_RS26780	699283..699759	-	477	WP_006479528.1	aromatic-ring-hydroxylating dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (16-107)

Antitoxin

• Uncharacterized protein HI1420 (6-93)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12616.44 Da
• Isoelectric Point: 8.9622

>T116199 WP_126367358.1 NZ_CP034547:694656-694994 [Burkholderia cenocepacia]
MVDIQQSVPYSAPTFSIRTTDVFDAWFAGLPDRLARRRIQARIDRLSMGNTGDWKSAGAPVVEMRIDHGPGYRVYYVRRG
TIWVILLCGGDKSTQRADIHAAHAMLAHLDME

Nucleotide

• Length: 339 bp

>T116199 NZ_CP034547:694656-694994 [Burkholderia cenocepacia]
ATGGTTGACATCCAGCAATCGGTGCCTTACAGTGCACCCACGTTCAGCATCCGGACCACCGACGTCTTCGACGCCTGGTT
TGCCGGCCTGCCCGATCGCCTCGCCCGGCGCCGCATCCAGGCGCGCATCGACCGCCTGTCGATGGGCAATACGGGCGACT
GGAAATCCGCCGGCGCCCCCGTCGTCGAAATGCGTATCGACCACGGCCCCGGCTATCGCGTCTATTACGTGAGGCGCGGA
ACGATATGGGTGATCCTGCTCTGCGGCGGCGATAAATCGACGCAACGGGCCGACATACACGCCGCGCACGCAATGCTCGC
GCACCTCGACATGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10593.97 Da
• Isoelectric Point: 6.0669

>AT116199 WP_050017389.1 NZ_CP034547:694998-695297 [Burkholderia cenocepacia]
MDKINTRPWDSAEHLKTEDDMAEYFDACLQEGGDDPAFIAHALGVIARARGMSQVARDAGLSREGLYKALSHDGNPSFGT
ILKVIKALGLQLHGAKAAA

Nucleotide

• Length: 300 bp

>AT116199 NZ_CP034547:694998-695297 [Burkholderia cenocepacia]
ATGGACAAGATCAACACGCGGCCGTGGGATTCGGCCGAACACCTGAAAACCGAAGACGACATGGCCGAGTACTTCGACGC
ATGCCTGCAGGAGGGCGGCGACGATCCGGCCTTCATCGCGCACGCGCTCGGCGTGATCGCCCGCGCACGCGGCATGTCGC
AGGTCGCGCGCGATGCGGGCCTGTCGCGCGAAGGGTTGTACAAGGCGCTGTCGCACGACGGCAACCCGAGCTTCGGCACG
ATCCTGAAAGTCATCAAGGCGCTCGGCCTGCAATTGCACGGCGCGAAGGCTGCCGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A228HNU1