Detailed information of TA system TA116192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): TscAT/-
• Location: 2514035..2514546
• Replicon: chromosome
• Accession: NZ_CP034486
• Organism: Staphylococcus aureus strain PMB 64-1

Toxin (Protein)

• Gene name: TscT
• Uniprot ID: X5IG98
• Locus tag: EJN87_RS12900
• Protein ID: WP_050961496.1
• Coordinates: 2514247..2514546 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: TscA
• Uniprot ID: X5IA75
• Locus tag: EJN87_RS12895
• Protein ID: WP_001058492.1
• Coordinates: 2514035..2514244 (+)
• Length: 70 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJN87_RS12855	2509103..2509606	+	504	WP_000934799.1	single-stranded DNA-binding protein	-
EJN87_RS12860	2509658..2509900	+	243	WP_000897044.1	30S ribosomal protein S18	-
EJN87_RS12865	2510016..2511122	-	1107	WP_000715303.1	Abi family protein	-
EJN87_RS12870	2511303..2512514	-	1212	WP_033854764.1	site-specific integrase	-
EJN87_RS12875	2512675..2513298	-	624	WP_033854765.1	transcriptional regulator	-
EJN87_RS12880	2513447..2513611	+	165	WP_033854775.1	helix-turn-helix domain-containing protein	-
EJN87_RS12885	2513612..2513884	+	273	WP_033854766.1	hypothetical protein	-
EJN87_RS12890	2513896..2514042	+	147	WP_000784888.1	hypothetical protein	-
EJN87_RS12895	2514035..2514244	+	210	WP_001058492.1	hypothetical protein	Antitoxin
EJN87_RS12900	2514247..2514546	+	300	WP_050961496.1	DUF1474 family protein	Toxin
EJN87_RS12905	2514621..2516234	+	1614	WP_033854769.1	hypothetical protein	-
EJN87_RS12910	2516405..2517208	+	804	WP_000148371.1	bifunctional DNA primase/polymerase	-
EJN87_RS12915	2517195..2517467	+	273	WP_001149387.1	hypothetical protein	-
EJN87_RS12920	2517469..2518110	+	642	WP_001019773.1	hypothetical protein	-
EJN87_RS12925	2518560..2518901	+	342	WP_001190606.1	hypothetical protein	-
EJN87_RS12930	2518913..2519491	+	579	WP_000846280.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2505040..2577458	72418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1474 (1-90)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11666.17 Da
• Isoelectric Point: 4.6685

>T116192 WP_050961496.1 NZ_CP034486:2514247-2514546 [Staphylococcus aureus]
MNWEIKDLMCDIEVIKEKINDVAIKHGWFVEDKFVKNKLETKQEHISYSAGYLEHRIQNEHTVELLQVYLKEFGELIQRF
HEIEKASSDVSLATESDDA

Nucleotide

• Length: 300 bp

>T116192 NZ_CP034486:2514247-2514546 [Staphylococcus aureus]
ATGAATTGGGAAATTAAAGATTTAATGTGTGATATTGAAGTGATAAAAGAAAAAATCAATGATGTAGCTATCAAACATGG
TTGGTTCGTTGAAGATAAATTTGTCAAAAATAAATTAGAAACAAAACAAGAACATATCAGTTACTCAGCAGGCTATTTAG
AACATCGTATACAAAATGAACATACAGTTGAGTTATTACAGGTGTACTTAAAAGAATTCGGTGAACTTATACAAAGATTT
CATGAAATAGAAAAAGCATCATCTGATGTAAGTTTGGCGACAGAATCAGATGACGCATAG

Antitoxin

Protein

• Length: 70 a.a.
• Molecular weight: 8238.28 Da
• Isoelectric Point: 4.3718

>AT116192 WP_001058492.1 NZ_CP034486:2514035-2514244 [Staphylococcus aureus]
MNNEQKEVIEHVVYQLELSVMNNLESYEHTEYVNGIEVVSEISREKHLELIMKWCAQELKNNFQLEKGE

Nucleotide

• Length: 210 bp

>AT116192 NZ_CP034486:2514035-2514244 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTAATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACTGAATATGTTAATGGTATTGAAGTGGTTTCAGAGATCAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Structures

Toxin

• AlphaFold DB: X5IG98

Antitoxin

• AlphaFold DB: X5IA75