Detailed information of TA system TA116165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/-
• Location: 1348045..1348961
• Replicon: chromosome
• Accession: NZ_CP034484
• Organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: EJJ34_RS07150
• Protein ID: WP_003244695.1
• Coordinates: 1348215..1348961 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: EJJ34_RS07145
• Protein ID: WP_003232646.1
• Coordinates: 1348045..1348215 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJJ34_RS07110 (EJJ34_07115)	1344908..1345237	+	330	WP_003232660.1	XkdW family protein	-
EJJ34_RS07115 (EJJ34_07120)	1345234..1345398	+	165	WP_003232658.1	XkdX family protein	-
EJJ34_RS07120 (EJJ34_07125)	1345442..1346281	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
EJJ34_RS07125 (EJJ34_07130)	1346334..1346603	+	270	WP_003232655.1	hemolysin XhlA family protein	-
EJJ34_RS07130 (EJJ34_07135)	1346616..1346879	+	264	WP_003232653.1	phage holin	-
EJJ34_RS07135 (EJJ34_07140)	1346892..1347785	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
EJJ34_RS07140 (EJJ34_07145)	1347822..1347959	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
EJJ34_RS07145 (EJJ34_07150)	1348045..1348215	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
EJJ34_RS07150 (EJJ34_07155)	1348215..1348961	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
EJJ34_RS07155 (EJJ34_07160)	1349071..1350072	-	1002	WP_065826289.1	inorganic phosphate transporter	-
EJJ34_RS07160 (EJJ34_07165)	1350085..1350702	-	618	WP_003218470.1	DUF47 domain-containing protein	-
EJJ34_RS07165 (EJJ34_07170)	1350978..1352294	-	1317	WP_003244819.1	serine/threonine exchanger	-
EJJ34_RS07170 (EJJ34_07175)	1352683..1353633	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
EJJ34_RS07175 (EJJ34_07180)	1353734..1353880	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (42-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (39-78)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 36664.99 Da
• Isoelectric Point: 5.5500

>T116165 WP_003244695.1 NZ_CP034484:c1348961-1348215 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFF

Nucleotide

• Length: 747 bp

>T116165 NZ_CP034484:c1348961-1348215 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
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Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 8407.08 Da
• Isoelectric Point: 5.5500

>AT116165 WP_003232646.1 NZ_CP034484:c1348215-1348045 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 171 bp

>AT116165 NZ_CP034484:c1348215-1348045 [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10]
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Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8