Detailed information of TA system TA116148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3549383..3550000
• Replicon: chromosome
• Accession: NZ_CP034482
• Organism: Rahnella aquatilis strain KM12

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: EJP81_RS16405
• Protein ID: WP_013576617.1
• Coordinates: 3549797..3550000 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: H8NYI6
• Locus tag: EJP81_RS16400
• Protein ID: WP_013576616.1
• Coordinates: 3549383..3549751 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP81_RS16370	3545373..3545627	+	255	WP_013576610.1	type B 50S ribosomal protein L31	-
EJP81_RS16375	3545648..3545788	+	141	WP_013576611.1	type B 50S ribosomal protein L36	-
EJP81_RS16380	3545913..3546791	-	879	WP_013576612.1	metal ABC transporter substrate-binding protein	-
EJP81_RS16385	3546835..3547692	-	858	WP_013576613.1	metal ABC transporter permease	-
EJP81_RS16390	3547689..3548417	-	729	WP_173362112.1	ABC transporter ATP-binding protein	-
EJP81_RS16395	3548800..3549153	+	354	WP_013576615.1	hypothetical protein	-
EJP81_RS16400	3549383..3549751	+	369	WP_013576616.1	Hha toxicity modulator TomB	Antitoxin
EJP81_RS16405	3549797..3550000	+	204	WP_013576617.1	hemolysin expression modulator Hha	Toxin
EJP81_RS16410	3550076..3551032	-	957	WP_013576618.1	LysR family transcriptional regulator	-
EJP81_RS16415	3551172..3552959	+	1788	WP_126125077.1	adenine deaminase	-
EJP81_RS16420	3553036..3554385	+	1350	WP_126125078.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T116148 WP_013576617.1 NZ_CP034482:3549797-3550000 [Rahnella aquatilis]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T116148 NZ_CP034482:3549797-3550000 [Rahnella aquatilis]
ATGAACAAAATCGACTATTTGATGCGTTTACGAAAATGCACCACTATCGATACACTTGAACGTGTTATTGAAAAAAATAA
GTACGAACTTTCCGACGACGAGCTGGAATTGTTTTACTCGGCAGCTGACCATCGTTTAGCGGAGCTAACGATGAACAAGT
TGTACGATAAAATCCCGGTATCAGTGTGGAAATTTGTACGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14391.15 Da
• Isoelectric Point: 4.7281

>AT116148 WP_013576616.1 NZ_CP034482:3549383-3549751 [Rahnella aquatilis]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT116148 NZ_CP034482:3549383-3549751 [Rahnella aquatilis]
ATGGATGAGTACTCGCCTAAGCGGCACGATATCGCTCAGTTACGCTACCTGAATGAAATGCTGTATGATGAAGGGATTGC
GACACTGGGTGACAGTCATCATGGTTGGGTTAACGATCCGACTTCGGCTGTTAACTTGCAGCTCAATGAACTGATTGAGC
ACATTGCATCCTTTGTGATGAGTTTTAAAATTAAGTATCCAGACGATAACCATTTAAGTGATCTGGTTGAAGAGTATCTG
GATGATACCTACACGCTGTTCAGTAATTATGGAATTAACGATTCTGATTTACGCCAATGGCAAAAGACCAAGAAGCGGTT
ATTCAGAATGTTCTCAGGGGACTACATCTGTACCCTAATGAAGACTTGA

Structures

Toxin

• AlphaFold DB: H8NYI7

Antitoxin

• AlphaFold DB: H8NYI6