Detailed information of TA system TA116136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 760410..761045
• Replicon: chromosome
• Accession: NZ_CP034482
• Organism: Rahnella aquatilis strain KM12

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: H8NSH0
• Locus tag: EJP81_RS03560
• Protein ID: WP_013574084.1
• Coordinates: 760863..761045 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: H8NSG9
• Locus tag: EJP81_RS03555
• Protein ID: WP_013574083.1
• Coordinates: 760410..760841 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP81_RS03530	755618..757072	-	1455	WP_126124629.1	efflux transporter outer membrane subunit	-
EJP81_RS03535	757096..757617	-	522	WP_013574079.1	transcriptional repressor MprA	-
EJP81_RS03540	757792..758130	-	339	WP_013574080.1	L-valine transporter subunit YgaH	-
EJP81_RS03545	758120..758881	-	762	WP_126124630.1	AzlC family ABC transporter permease	-
EJP81_RS03550	759056..760240	-	1185	WP_126124631.1	MFS transporter	-
EJP81_RS03555	760410..760841	-	432	WP_013574083.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
EJP81_RS03560	760863..761045	-	183	WP_013574084.1	type II toxin-antitoxin system HicA family toxin	Toxin
EJP81_RS03565	761162..762157	-	996	WP_013574085.1	glycine betaine/L-proline ABC transporter substrate-binding protein ProX	-
EJP81_RS03570	762182..763192	-	1011	WP_112152327.1	glycine betaine/L-proline ABC transporter substrate-binding protein ProX	-
EJP81_RS03575	763298..764422	-	1125	WP_187514584.1	glycine betaine/L-proline ABC transporter permease ProW	-
EJP81_RS03580	764494..765690	-	1197	WP_013574088.1	glycine betaine/L-proline ABC transporter ATP-binding protein ProV	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (11-137)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6646.89 Da
• Isoelectric Point: 11.1595

>T116136 WP_013574084.1 NZ_CP034482:c761045-760863 [Rahnella aquatilis]
MKSTELIKALLAAGCELKRHRGGSHQIWWSPVTNKTFPVPHPKNDLPVGTLKSIRKMAGI

Nucleotide

• Length: 183 bp

>T116136 NZ_CP034482:c761045-760863 [Rahnella aquatilis]
ATGAAATCAACGGAATTGATTAAAGCGCTGCTTGCAGCGGGTTGTGAACTCAAGCGTCACCGGGGAGGAAGTCATCAAAT
TTGGTGGTCACCTGTCACCAACAAAACCTTTCCCGTTCCCCACCCCAAAAATGATTTACCTGTGGGAACTCTCAAATCCA
TTAGAAAAATGGCAGGGATATGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 16317.52 Da
• Isoelectric Point: 4.4314

>AT116136 WP_013574083.1 NZ_CP034482:c760841-760410 [Rahnella aquatilis]
MFFSVGLETPKDDKTAYGMVVPAFTAFDYGCFSAADTREEIAPMVREAILMTVEDLIESERFVVDDIFDAGHLVYAANPE
YADFDTWFLIDIDLSEFEGKAHRINISLPDTLIKRIDNKVKNHPSYRDRSHFLAMAARKELLT

Nucleotide

• Length: 432 bp

>AT116136 NZ_CP034482:c760841-760410 [Rahnella aquatilis]
ATGTTCTTTTCAGTCGGTCTTGAAACACCAAAAGATGACAAAACCGCTTACGGTATGGTGGTACCTGCATTTACCGCGTT
TGATTACGGATGCTTCTCTGCAGCGGATACCCGTGAAGAAATCGCCCCTATGGTTCGCGAAGCCATCCTGATGACAGTAG
AAGATTTGATCGAAAGCGAACGCTTTGTTGTTGACGATATTTTTGATGCAGGCCATTTGGTGTATGCCGCTAATCCCGAA
TATGCCGACTTCGATACCTGGTTTTTGATTGATATTGATCTCTCGGAATTCGAAGGAAAAGCGCATCGAATCAATATTTC
GCTGCCTGACACCCTGATCAAACGCATTGATAATAAAGTTAAAAATCATCCGTCATATCGCGACCGCAGCCATTTTCTGG
CGATGGCCGCACGTAAAGAATTGCTGACCTGA

Structures

Toxin

• AlphaFold DB: H8NSH0

Antitoxin

• AlphaFold DB: H8NSG9