Detailed information of TA system TA116134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 28262..28784
• Replicon: chromosome
• Accession: NZ_CP034482
• Organism: Rahnella aquatilis strain KM12

Toxin (Protein)

• Gene name: relE
• Uniprot ID: H8NLY9
• Locus tag: EJP81_RS00130
• Protein ID: WP_013573377.1
• Coordinates: 28500..28784 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: H8NLY8
• Locus tag: EJP81_RS00125
• Protein ID: WP_013573376.1
• Coordinates: 28262..28510 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP81_RS00105	24090..24752	-	663	WP_037033864.1	OmpA family lipoprotein	-
EJP81_RS00110	24974..25417	-	444	WP_013573373.1	N-acetyltransferase	-
EJP81_RS00115	25414..25986	-	573	WP_037033862.1	DNA-3-methyladenine glycosylase I	-
EJP81_RS00120	26177..28183	+	2007	WP_013573375.1	autotransporter domain-containing esterase	-
EJP81_RS00125	28262..28510	+	249	WP_013573376.1	plasmid stabilization protein	Antitoxin
EJP81_RS00130	28500..28784	+	285	WP_013573377.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
EJP81_RS00135	28906..29820	+	915	WP_095924009.1	glycine--tRNA ligase subunit alpha	-
EJP81_RS00140	29830..31899	+	2070	WP_013573379.1	glycine--tRNA ligase subunit beta	-
EJP81_RS00145	32110..32832	+	723	WP_013573380.1	DUF3053 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10954.92 Da
• Isoelectric Point: 10.5358

>T116134 WP_013573377.1 NZ_CP034482:28500-28784 [Rahnella aquatilis]
MTYKLNFFKSALKEWKALAPTIKEQFKKKLAERLENPHVVSARLCGPKNNRYKIKLKSLGYRLVYQVEDQEIIVTVVAVG
KREGNEAYKLAEKR

Nucleotide

• Length: 285 bp

>T116134 NZ_CP034482:28500-28784 [Rahnella aquatilis]
ATGACTTATAAACTCAATTTTTTCAAATCAGCTTTAAAAGAATGGAAAGCACTTGCCCCAACGATCAAGGAGCAATTTAA
AAAGAAACTGGCCGAGCGGCTGGAGAATCCTCACGTGGTTTCTGCCCGCTTATGTGGCCCCAAAAATAACCGCTATAAAA
TCAAATTAAAGAGTCTTGGTTATCGCCTGGTGTATCAGGTAGAAGATCAGGAAATCATCGTGACCGTTGTCGCGGTGGGT
AAACGCGAAGGCAACGAAGCGTATAAACTGGCTGAGAAGCGCTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9155.35 Da
• Isoelectric Point: 3.9951

>AT116134 WP_013573376.1 NZ_CP034482:28262-28510 [Rahnella aquatilis]
MPFEILTSSAASITELKRDPMGTFSAGEGEAVAILNRNEPAFYCVPPDVYAYFLSLAEDVELNRIMDARQGEERIRLTLD
DL

Nucleotide

• Length: 249 bp

>AT116134 NZ_CP034482:28262-28510 [Rahnella aquatilis]
ATGCCGTTTGAAATACTCACTTCCTCAGCCGCCAGTATTACTGAACTAAAACGCGACCCGATGGGAACATTTAGTGCAGG
CGAAGGCGAAGCGGTGGCCATACTCAATCGTAACGAGCCCGCTTTTTATTGCGTTCCGCCCGATGTTTACGCCTATTTTC
TGAGTCTTGCAGAAGACGTTGAATTGAACCGCATAATGGATGCGCGGCAGGGTGAAGAAAGGATAAGGCTGACACTGGAT
GACTTATAA

Structures

Toxin

• AlphaFold DB: H8NLY9

Antitoxin

• AlphaFold DB: H8NLY8