Detailed information of TA system TA116132

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3548686..3549303
• Replicon: chromosome
• Accession: NZ_CP034481
• Organism: Rahnella aquatilis strain KM25

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: H8NYI7
• Locus tag: EJP79_RS16405
• Protein ID: WP_013576617.1
• Coordinates: 3549100..3549303 (+)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: H8NYI6
• Locus tag: EJP79_RS16400
• Protein ID: WP_013576616.1
• Coordinates: 3548686..3549054 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJP79_RS16370	3544676..3544930	+	255	WP_013576610.1	type B 50S ribosomal protein L31	-
EJP79_RS16375	3544951..3545091	+	141	WP_013576611.1	type B 50S ribosomal protein L36	-
EJP79_RS16380	3545216..3546094	-	879	WP_013576612.1	metal ABC transporter substrate-binding protein	-
EJP79_RS16385	3546138..3546995	-	858	WP_013576613.1	metal ABC transporter permease	-
EJP79_RS16390	3546992..3547720	-	729	WP_173362112.1	ABC transporter ATP-binding protein	-
EJP79_RS16395	3548103..3548456	+	354	WP_013576615.1	hypothetical protein	-
EJP79_RS16400	3548686..3549054	+	369	WP_013576616.1	Hha toxicity modulator TomB	Antitoxin
EJP79_RS16405	3549100..3549303	+	204	WP_013576617.1	hemolysin expression modulator Hha	Toxin
EJP79_RS16410	3549379..3550335	-	957	WP_013576618.1	LysR family transcriptional regulator	-
EJP79_RS16415	3550475..3552262	+	1788	WP_126125077.1	adenine deaminase	-
EJP79_RS16420	3552339..3553688	+	1350	WP_126125078.1	NCS2 family permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-118)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8114.45 Da
• Isoelectric Point: 6.9764

>T116132 WP_013576617.1 NZ_CP034481:3549100-3549303 [Rahnella aquatilis]
MNKIDYLMRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPVSVWKFVR

Nucleotide

• Length: 204 bp

>T116132 NZ_CP034481:3549100-3549303 [Rahnella aquatilis]
ATGAACAAAATCGACTATTTGATGCGTTTACGAAAATGCACCACTATCGATACACTTGAACGTGTTATTGAAAAAAATAA
GTACGAACTTTCCGACGACGAGCTGGAATTGTTTTACTCGGCAGCTGACCATCGTTTAGCGGAGCTAACGATGAACAAGT
TGTACGATAAAATCCCGGTATCAGTGTGGAAATTTGTACGCTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14391.15 Da
• Isoelectric Point: 4.7281

>AT116132 WP_013576616.1 NZ_CP034481:3548686-3549054 [Rahnella aquatilis]
MDEYSPKRHDIAQLRYLNEMLYDEGIATLGDSHHGWVNDPTSAVNLQLNELIEHIASFVMSFKIKYPDDNHLSDLVEEYL
DDTYTLFSNYGINDSDLRQWQKTKKRLFRMFSGDYICTLMKT

Nucleotide

• Length: 369 bp

>AT116132 NZ_CP034481:3548686-3549054 [Rahnella aquatilis]
ATGGATGAGTACTCGCCTAAGCGGCACGATATCGCTCAGTTACGCTACCTGAATGAAATGCTGTATGATGAAGGGATTGC
GACACTGGGTGACAGTCATCATGGTTGGGTTAACGATCCGACTTCGGCTGTTAACTTGCAGCTCAATGAACTGATTGAGC
ACATTGCATCCTTTGTGATGAGTTTTAAAATTAAGTATCCAGACGATAACCATTTAAGTGATCTGGTTGAAGAGTATCTG
GATGATACCTACACGCTGTTCAGTAATTATGGAATTAACGATTCTGATTTACGCCAATGGCAAAAGACCAAGAAGCGGTT
ATTCAGAATGTTCTCAGGGGACTACATCTGTACCCTAATGAAGACTTGA

Structures

Toxin

• AlphaFold DB: H8NYI7

Antitoxin

• AlphaFold DB: H8NYI6