Detailed information of TA system TA116116

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 153078..153604
• Replicon: plasmid pCFSAN047154_2
• Accession: NZ_CP034476
• Organism: Pantoea agglomerans strain CFSAN047154

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A7X2SUG9
• Locus tag: D1629_RS22750
• Protein ID: WP_033759930.1
• Coordinates: 153317..153604 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A8X8DPB7
• Locus tag: D1629_RS22745
• Protein ID: WP_010257033.1
• Coordinates: 153078..153317 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1629_RS22730	148906..149574	+	669	WP_033774070.1	hypothetical protein	-
D1629_RS22735	149624..150640	-	1017	WP_010257039.1	NAD(P)-dependent alcohol dehydrogenase	-
D1629_RS22740	150745..152718	-	1974	WP_129063440.1	FIST C-terminal domain-containing protein	-
D1629_RS22745	153078..153317	+	240	WP_010257033.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
D1629_RS22750	153317..153604	+	288	WP_033759930.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1629_RS22755	153649..153945	-	297	WP_039387382.1	hypothetical protein	-
D1629_RS22760	154145..154321	+	177	WP_010257023.1	stress-induced protein	-
D1629_RS22765	154385..155281	-	897	WP_129063441.1	LysR family transcriptional regulator	-
D1629_RS22770	155415..156608	+	1194	WP_164973036.1	MFS transporter	-
D1629_RS22775	156677..157702	+	1026	WP_010257012.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	pilW	1..211326	211326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-77)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11076.95 Da
• Isoelectric Point: 10.3173

>T116116 WP_033759930.1 NZ_CP034476:153317-153604 [Pantoea agglomerans]
MTWQLEFDQRALKEWRKLGPTVREQLKKKLAECLESPRIEANKLSGMPDCYKIKLKSSGYRLVYQVIDDRVVVFVVAVGK
RERSEVYSAASRRLS

Nucleotide

• Length: 288 bp

>T116116 NZ_CP034476:153317-153604 [Pantoea agglomerans]
ATGACCTGGCAACTGGAGTTCGATCAGAGGGCACTGAAAGAGTGGCGCAAGCTGGGCCCGACAGTACGTGAACAGCTTAA
AAAGAAGCTGGCTGAGTGTCTTGAGTCACCCAGAATTGAAGCGAACAAACTCAGCGGGATGCCTGACTGCTATAAGATCA
AGCTAAAGTCATCCGGTTATCGACTTGTATACCAGGTTATTGACGACCGGGTGGTGGTTTTTGTGGTGGCTGTCGGGAAA
AGAGAGCGCTCAGAGGTTTATTCTGCCGCTTCCAGGCGTCTCTCTTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9002.33 Da
• Isoelectric Point: 4.3410

>AT116116 WP_010257033.1 NZ_CP034476:153078-153317 [Pantoea agglomerans]
MGTINIRIDDDLKTRSYAALEKMGVTPSDALRLMLEYIADNERLPFKQTLLSDEDAALVDIVKERLRNPEPVRVTLDDL

Nucleotide

• Length: 240 bp

>AT116116 NZ_CP034476:153078-153317 [Pantoea agglomerans]
ATGGGCACAATTAACATTCGAATCGATGATGACCTGAAGACACGATCCTATGCTGCGCTCGAAAAAATGGGTGTGACTCC
TTCTGATGCGCTGCGCCTGATGCTTGAATATATTGCAGATAATGAACGTCTTCCCTTTAAGCAAACGTTACTCAGTGACG
AAGATGCGGCGTTAGTAGACATAGTTAAAGAACGCCTGCGAAACCCTGAGCCTGTCCGGGTCACACTGGATGACCTTTAA

Structures

Toxin

• AlphaFold DB: A0A7X2SUG9