116096

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1020817..1021434 Replicon chromosome
Accession NZ_CP034469
Organism Pantoea agglomerans strain CFSAN047153

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag D1628_RS04645 Protein ID WP_003850458.1
Coordinates 1020817..1021032 (-) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E0LTU8
Locus tag D1628_RS04650 Protein ID WP_003850455.1
Coordinates 1021057..1021434 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D1628_RS04615 1016738..1017076 + 339 WP_003850469.1 P-II family nitrogen regulator -
D1628_RS04620 1017111..1018397 + 1287 WP_010255877.1 ammonium transporter AmtB -
D1628_RS04625 1018448..1019311 - 864 WP_010255880.1 acyl-CoA thioesterase II -
D1628_RS04630 1019502..1020059 + 558 WP_010255881.1 YbaY family lipoprotein -
D1628_RS04635 1020099..1020410 - 312 WP_033756781.1 MGMT family protein -
D1628_RS04645 1020817..1021032 - 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
D1628_RS04650 1021057..1021434 - 378 WP_003850455.1 Hha toxicity modulator TomB Antitoxin
D1628_RS04655 1021582..1021935 - 354 WP_010255885.1 hypothetical protein -
D1628_RS04660 1022277..1022945 + 669 WP_033772778.1 ABC transporter ATP-binding protein -
D1628_RS04665 1022942..1023781 + 840 WP_010255890.1 metal ABC transporter permease -
D1628_RS04670 1023797..1024675 + 879 WP_033771082.1 metal ABC transporter substrate-binding protein -
D1628_RS04675 1024721..1024861 - 141 WP_010255896.1 type B 50S ribosomal protein L36 -
D1628_RS04680 1024873..1025127 - 255 WP_010671219.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T116096 WP_003850458.1 NZ_CP034469:c1021032-1020817 [Pantoea agglomerans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T116096 NZ_CP034469:c1021032-1020817 [Pantoea agglomerans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGCTGCCGCTCTCTTGATACCCTGGAAAGGGTGAT
TGAAAAAAATAAGTATGAATTACCGGAAGATGAATTAGCGGTATTTTACTCAGCAGCTGACCATCGCCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCGGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14636.25 Da        Isoelectric Point: 4.3976

>AT116096 WP_003850455.1 NZ_CP034469:c1021434-1021057 [Pantoea agglomerans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGVNSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT116096 NZ_CP034469:c1021434-1021057 [Pantoea agglomerans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATACCTTTGTGAGAGTCTGTTTGACGACAGCATGGC
AACATTAACCGACAGTCATCATGGCTGGGTTAATGATCCCACTTCTGAAAGCAATCTACAGCTTAACGATTTGATTGAGC
ATATCGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAATTATGGTGTCAATAGTCCGGATCTTCAGCGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCGAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A1I5AG55

References