Detailed information of TA system TA116084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1147323..1148340
• Replicon: chromosome
• Accession: NZ_CP034449
• Organism: Mesorhizobium sp. M2A.F.Ca.ET.046.03.2.1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EJ072_RS05555
• Protein ID: WP_210211652.1
• Coordinates: 1147323..1147898 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: EJ072_RS05560
• Protein ID: WP_126078907.1
• Coordinates: 1147894..1148340 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ072_RS05545	1143868..1144386	+	519	WP_189343224.1	Mov34/MPN/PAD-1 family protein	-
EJ072_RS05550	1144677..1146161	-	1485	WP_126078905.1	hypothetical protein	-
EJ072_RS35825	1147144..1147290	-	147	WP_189343225.1	hypothetical protein	-
EJ072_RS05555	1147323..1147898	-	576	WP_210211652.1	PIN domain-containing protein	Toxin
EJ072_RS05560	1147894..1148340	-	447	WP_126078907.1	helix-turn-helix domain-containing protein	Antitoxin
EJ072_RS05565	1148844..1149022	-	179	Protein_1093	cold-shock protein	-
EJ072_RS05570	1149031..1149996	-	966	WP_126078908.1	DUF4238 domain-containing protein	-
EJ072_RS05575	1150227..1150406	-	180	Protein_1095	aldehyde-activating protein	-
EJ072_RS05580	1150474..1152036	-	1563	WP_126078909.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1140603..1159227	18624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (78-124)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21909.17 Da
• Isoelectric Point: 5.6593

>T116084 WP_210211652.1 NZ_CP034449:c1147898-1147323 [Mesorhizobium sp. M2A.F.Ca.ET.046.03.2.1]
IRESSYTVILDACVLYPAPLRDFLMELAAGGLFRAKWTEQIHDEWIRNVLKNREDLKPEQLLRTKQLMNQAVPDCTVEGY
EDLIEGLKLPDGDDRHVLAAAIHAACDAIITFNLKDFPRDYLAKFQIELLHPDEFLFHQFGLNTAAVVVAAQRCRKRLKN
PPRTAEEYLDTLERQGLTKTVTELRNYASVI

Nucleotide

• Length: 576 bp

>T116084 NZ_CP034449:c1147898-1147323 [Mesorhizobium sp. M2A.F.Ca.ET.046.03.2.1]
ATTAGGGAGTCCAGTTACACCGTCATTCTCGACGCTTGCGTATTATACCCGGCCCCGCTGCGAGACTTCCTGATGGAGCT
TGCAGCGGGAGGGCTATTTCGCGCGAAATGGACTGAACAAATCCATGACGAATGGATTCGCAACGTGCTTAAGAATCGCG
AGGACCTTAAGCCCGAGCAGCTTTTGCGGACAAAACAACTGATGAACCAGGCAGTGCCTGACTGCACGGTTGAAGGCTAC
GAGGACCTGATTGAGGGTCTAAAACTACCTGACGGTGACGACAGACATGTTCTCGCAGCCGCTATCCATGCGGCTTGTGA
CGCCATCATCACTTTCAATCTGAAAGACTTTCCGCGTGACTATCTCGCCAAGTTCCAGATAGAGCTTCTGCATCCGGACG
AATTTCTGTTCCATCAGTTTGGCCTGAATACAGCCGCCGTCGTGGTAGCGGCCCAGCGTTGTCGTAAACGTTTGAAAAAC
CCTCCGAGAACGGCAGAGGAATACCTCGATACCCTCGAGCGGCAAGGGCTCACCAAAACGGTGACCGAACTCCGAAACTA
CGCCTCGGTCATTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16841.17 Da
• Isoelectric Point: 5.5937

>AT116084 WP_126078907.1 NZ_CP034449:c1148340-1147894 [Mesorhizobium sp. M2A.F.Ca.ET.046.03.2.1]
MTTLLERPILPSADDAELAAEASRHLSRAKHEDAELRIQVDGGEMLRLPKAVNDLLYHLLTEMAQGNAVTLFPIHAELTT
QEAADYLNVSRPYLVRLLEEGKVKFHMVGTHRRVKFRDLDAFRRSTEEERQRVMDELAAQAQDLGMGY

Nucleotide

• Length: 447 bp

>AT116084 NZ_CP034449:c1148340-1147894 [Mesorhizobium sp. M2A.F.Ca.ET.046.03.2.1]
ATGACTACGCTGCTCGAACGACCGATATTGCCTTCGGCCGACGATGCCGAATTGGCTGCGGAGGCCAGCCGCCACCTTTC
TAGAGCCAAGCACGAGGATGCTGAATTGCGCATCCAAGTGGATGGGGGAGAGATGCTGCGCCTGCCTAAGGCAGTGAACG
ATCTTCTCTATCATTTGCTCACCGAAATGGCACAGGGTAATGCCGTTACCCTGTTCCCAATCCATGCCGAGCTGACCACA
CAGGAGGCTGCTGACTACCTGAATGTGTCACGTCCTTATCTTGTTCGACTCCTCGAGGAGGGTAAGGTCAAGTTCCATAT
GGTAGGAACTCATCGACGGGTCAAATTCCGAGATTTAGATGCATTCCGTAGGTCAACTGAGGAAGAGCGTCAGCGCGTAA
TGGATGAACTCGCAGCCCAAGCGCAGGATTTGGGGATGGGCTATTAG