Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 2849721..2850316 Replicon chromosome
Accession NZ_CP034446
Organism Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1

Toxin (Protein)


Gene name PumA Uniprot ID A0A5E8ES58
Locus tag EJ069_RS14210 Protein ID WP_042637956.1
Coordinates 2850029..2850316 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID -
Locus tag EJ069_RS14205 Protein ID WP_126061821.1
Coordinates 2849721..2850026 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EJ069_RS14190 2846919..2847836 + 918 WP_126061818.1 glycoside hydrolase family 26 protein -
EJ069_RS14195 2847967..2848467 + 501 WP_126061819.1 DUF995 domain-containing protein -
EJ069_RS14200 2848558..2849715 + 1158 WP_126061820.1 hypothetical protein -
EJ069_RS14205 2849721..2850026 - 306 WP_126061821.1 putative addiction module antidote protein Antitoxin
EJ069_RS14210 2850029..2850316 - 288 WP_042637956.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
EJ069_RS14215 2850369..2851364 - 996 WP_126061822.1 Rieske 2Fe-2S domain-containing protein -
EJ069_RS14220 2851361..2852527 - 1167 WP_210211332.1 GNAT family N-acetyltransferase -
EJ069_RS14225 2852503..2853759 - 1257 WP_126061824.1 CoF synthetase -
EJ069_RS14230 2853756..2854580 - 825 WP_126061825.1 MBL fold metallo-hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(3-94)

Antitoxin

Uncharacterized protein HI1420

(5-91)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 10659.39 Da        Isoelectric Point: 10.2219

>T116068 WP_042637956.1 NZ_CP034446:c2850316-2850029 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
MIEVRQTADFANWLDGLRDAAARLRIAARIRRVEIGNIGDVKYFDGIGELRIDHGPGYRLYFVKIGNAVIILLCGGDKSS
QRRDIAKAKQMAKEI

Download         Length: 288 bp

>T116068 NZ_CP034446:c2850316-2850029 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
ATGATCGAGGTCCGGCAGACAGCCGATTTCGCCAACTGGCTGGACGGACTTCGCGATGCTGCGGCTCGCCTGCGCATCGC
TGCCCGCATTCGTCGGGTCGAAATCGGCAACATTGGTGATGTCAAATATTTCGATGGCATCGGCGAACTTCGGATCGACC
ATGGCCCAGGCTACCGGCTCTACTTTGTGAAGATCGGAAACGCCGTGATCATCCTGCTCTGTGGCGGCGACAAGTCCAGC
CAAAGGCGCGACATCGCCAAGGCGAAACAAATGGCAAAGGAGATATGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 10682.29 Da        Isoelectric Point: 4.7824

>AT116068 WP_126061821.1 NZ_CP034446:c2850026-2849721 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
MPLETTRFDILDYLKTPEDRLAYIEAAFEDGDPALITHALGDVARSIGMTAVAKNAGVTREALYKALSEKGDPRLSTLLG
VTKALGLQLDVKPIGPGSLGA

Download         Length: 306 bp

>AT116068 NZ_CP034446:c2850026-2849721 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
ATGCCTCTCGAAACCACCCGCTTCGACATTCTCGATTACCTGAAAACCCCGGAGGATCGCTTGGCCTACATCGAGGCCGC
TTTCGAGGATGGTGATCCTGCGCTCATAACGCATGCGTTGGGCGACGTAGCGCGCTCCATAGGCATGACGGCCGTGGCTA
AGAACGCCGGTGTCACGCGGGAGGCGCTCTACAAGGCATTGAGCGAGAAGGGTGATCCGCGCCTCAGCACACTGCTGGGT
GTCACCAAGGCACTTGGTCTGCAACTTGACGTGAAACCTATCGGCCCTGGCTCGCTCGGGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E8ES58

Antitoxin

Source ID Structure

References