Detailed information of TA system TA116067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2216309..2216879
• Replicon: chromosome
• Accession: NZ_CP034446
• Organism: Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: EJ069_RS10730
• Protein ID: WP_126061253.1
• Coordinates: 2216622..2216879 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: EJ069_RS10725
• Protein ID: WP_126061252.1
• Coordinates: 2216309..2216635 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ069_RS10685	2211472..2212413	+	942	WP_126061246.1	metal ABC transporter substrate-binding protein	-
EJ069_RS10690	2212545..2212967	+	423	WP_126061247.1	YeeE/YedE family protein	-
EJ069_RS10695	2212971..2213408	+	438	WP_126061248.1	YeeE/YedE family protein	-
EJ069_RS10700	2213500..2213838	+	339	WP_042646282.1	TIGR01244 family phosphatase	-
EJ069_RS10705	2213903..2214370	-	468	WP_042646286.1	Lrp/AsnC family transcriptional regulator	-
EJ069_RS10710	2214508..2215623	+	1116	WP_126061249.1	4-hydroxyphenylpyruvate dioxygenase	-
EJ069_RS10715	2215694..2215918	+	225	WP_126061250.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
EJ069_RS10720	2215915..2216292	+	378	WP_126061251.1	type II toxin-antitoxin system VapC family toxin	-
EJ069_RS10725	2216309..2216635	-	327	WP_126061252.1	BrnA antitoxin family protein	Antitoxin
EJ069_RS10730	2216622..2216879	-	258	WP_126061253.1	BrnT family toxin	Toxin
EJ069_RS10735	2216936..2217472	-	537	WP_126061254.1	DinB family protein	-
EJ069_RS10740	2217501..2218514	-	1014	WP_126061255.1	fumarylacetoacetate hydrolase family protein	-
EJ069_RS10745	2218511..2219410	-	900	WP_126061256.1	MBL fold metallo-hydrolase	-
EJ069_RS10750	2219407..2220720	-	1314	WP_126061257.1	homogentisate 1,2-dioxygenase	-
EJ069_RS10755	2220852..2221274	+	423	Protein_2114	MarR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-77)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (31-104)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9437.01 Da
• Isoelectric Point: 10.2947

>T116067 WP_126061253.1 NZ_CP034446:c2216879-2216622 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
MKIVWDEPKRLANIEKHGLDFAVLDEEFFLASTIRVAKAGRFMAIGRVVGSVVAVVFARLGSEGISIISMRLANQTERRL
FNGES

Nucleotide

• Length: 258 bp

>T116067 NZ_CP034446:c2216879-2216622 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
ATGAAGATCGTTTGGGACGAACCGAAACGGTTGGCCAATATCGAGAAGCATGGGCTGGATTTCGCCGTTCTGGACGAGGA
GTTCTTTCTGGCTTCGACGATCCGGGTGGCCAAAGCTGGCCGCTTCATGGCGATTGGTCGGGTAGTCGGTAGCGTTGTTG
CGGTGGTCTTTGCCCGGCTTGGCTCCGAAGGCATCTCCATCATTTCAATGCGGCTGGCCAACCAGACGGAAAGGAGATTG
TTCAATGGCGAAAGCTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12285.90 Da
• Isoelectric Point: 10.4136

>AT116067 WP_126061252.1 NZ_CP034446:c2216635-2216309 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
MAKAKLSIMKPDKDFQPGKGFTKEDWDAVSDNPEWTEEDFRNARPFTEVFPDLAESIRRSRGRPALDNPKKQVTLRLDSD
VVARFRAGGPGWQSRINDILRKAAGLQK

Nucleotide

• Length: 327 bp

>AT116067 NZ_CP034446:c2216635-2216309 [Mesorhizobium sp. M2A.F.Ca.ET.043.05.1.1]
ATGGCGAAAGCTAAGCTGAGCATCATGAAACCCGACAAGGATTTTCAACCAGGCAAGGGCTTTACCAAGGAAGATTGGGA
CGCGGTTTCGGACAATCCCGAATGGACCGAGGAGGATTTTCGAAACGCCCGCCCCTTCACGGAAGTGTTCCCGGACCTCG
CCGAAAGCATCCGCCGTTCGCGCGGCCGACCTGCGCTCGACAACCCGAAGAAGCAGGTGACGCTGCGTCTCGATAGCGAC
GTGGTCGCCCGCTTCCGCGCCGGCGGACCCGGCTGGCAGAGCCGCATCAACGACATTCTGCGGAAAGCCGCCGGCCTCCA
AAAGTAG