Detailed information of TA system TA116060

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 1991697..1992714
• Replicon: chromosome
• Accession: NZ_CP034444
• Organism: Mesorhizobium sp. M1D.F.Ca.ET.043.01.1.1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: EJ067_RS09905
• Protein ID: WP_210211708.1
• Coordinates: 1991697..1992272 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: -
• Locus tag: EJ067_RS09910
• Protein ID: WP_126078907.1
• Coordinates: 1992268..1992714 (-)
• Length: 149 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ067_RS09895	1988242..1988760	+	519	WP_189343224.1	Mov34/MPN/PAD-1 family protein	-
EJ067_RS09900	1989051..1990535	-	1485	WP_126078905.1	hypothetical protein	-
EJ067_RS09905	1991697..1992272	-	576	WP_210211708.1	PIN domain-containing protein	Toxin
EJ067_RS09910	1992268..1992714	-	447	WP_126078907.1	helix-turn-helix domain-containing protein	Antitoxin
EJ067_RS09915	1993218..1993396	-	179	Protein_1946	cold-shock protein	-
EJ067_RS09920	1993405..1994370	-	966	WP_126078908.1	DUF4238 domain-containing protein	-
EJ067_RS09925	1994601..1994780	-	180	Protein_1948	aldehyde-activating protein	-
EJ067_RS09930	1994848..1996410	-	1563	WP_126078909.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1984977..2003601	18624

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• Helix-turn-helix domain, group 17 (78-124)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21908.22 Da
• Isoelectric Point: 6.3382

>T116060 WP_210211708.1 NZ_CP034444:c1992272-1991697 [Mesorhizobium sp. M1D.F.Ca.ET.043.01.1.1]
IRKSSYTVILDACVLYPAPLRDFLMELAAGGLFRAKWTEQIHDEWIRNVLKNREDLKPEQLLRTKQLMNQAVPDCTVEGY
EDLIEGLKLPDGDDRHVLAAAIHAACDAIITFNLKDFPRDYLAKFQIELLHPDEFLFHQFGLNTAAVVVAAQRCRKRLKN
PPRTAEEYLDTLERQGLTKTVTELRNYASVI

Nucleotide

• Length: 576 bp

>T116060 NZ_CP034444:c1992272-1991697 [Mesorhizobium sp. M1D.F.Ca.ET.043.01.1.1]
ATTAGGAAGTCCAGTTACACCGTCATTCTCGACGCTTGCGTATTATACCCGGCCCCGCTGCGAGACTTCCTGATGGAGCT
TGCAGCGGGAGGGCTATTTCGCGCGAAATGGACTGAACAAATCCATGACGAATGGATTCGCAACGTGCTTAAGAATCGCG
AGGACCTTAAGCCCGAGCAGCTTTTGCGGACAAAACAACTGATGAACCAGGCAGTGCCTGACTGCACGGTTGAAGGCTAC
GAGGACCTGATTGAGGGTCTAAAACTACCTGACGGTGACGACAGACATGTTCTCGCAGCCGCTATCCATGCGGCTTGTGA
CGCCATCATCACTTTCAATCTGAAAGACTTTCCGCGTGACTATCTCGCCAAGTTCCAGATAGAGCTTCTGCATCCGGACG
AATTTCTGTTCCATCAGTTTGGCCTGAATACAGCCGCCGTCGTGGTAGCGGCCCAGCGTTGTCGTAAACGTTTGAAAAAC
CCTCCGAGAACGGCAGAGGAATACCTCGATACCCTCGAGCGGCAAGGGCTCACCAAAACGGTGACCGAACTCCGAAACTA
CGCCTCGGTCATTTAG

Antitoxin

Protein

• Length: 149 a.a.
• Molecular weight: 16841.17 Da
• Isoelectric Point: 5.5937

>AT116060 WP_126078907.1 NZ_CP034444:c1992714-1992268 [Mesorhizobium sp. M1D.F.Ca.ET.043.01.1.1]
MTTLLERPILPSADDAELAAEASRHLSRAKHEDAELRIQVDGGEMLRLPKAVNDLLYHLLTEMAQGNAVTLFPIHAELTT
QEAADYLNVSRPYLVRLLEEGKVKFHMVGTHRRVKFRDLDAFRRSTEEERQRVMDELAAQAQDLGMGY

Nucleotide

• Length: 447 bp

>AT116060 NZ_CP034444:c1992714-1992268 [Mesorhizobium sp. M1D.F.Ca.ET.043.01.1.1]
ATGACTACGCTGCTCGAACGACCGATATTGCCTTCGGCCGACGATGCCGAATTGGCTGCGGAGGCCAGCCGCCACCTTTC
TAGAGCCAAGCACGAGGATGCTGAATTGCGCATCCAAGTGGATGGGGGAGAGATGCTGCGCCTGCCTAAGGCAGTGAACG
ATCTTCTCTATCATTTGCTCACCGAAATGGCACAGGGTAATGCCGTTACCCTGTTCCCAATCCATGCCGAGCTGACCACA
CAGGAGGCTGCTGACTACCTGAATGTGTCACGTCCTTATCTTGTTCGACTCCTCGAGGAGGGTAAGGTCAAGTTCCATAT
GGTAGGAACTCATCGACGGGTCAAATTCCGAGATTTAGATGCATTCCGTAGGTCAACTGAGGAAGAGCGTCAGCGCGTAA
TGGATGAACTCGCAGCCCAAGCGCAGGATTTGGGGATGGGCTATTAG