Detailed information of TA system TA116055

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-Phd
• Location: 1111827..1112518
• Replicon: chromosome
• Accession: NZ_CP034443
• Organism: Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EJ066_RS05610
• Protein ID: WP_126035670.1
• Coordinates: 1112066..1112518 (+)
• Length: 151 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EJ066_RS05605
• Protein ID: WP_126035668.1
• Coordinates: 1111827..1112069 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ066_RS05580	1107325..1108209	-	885	WP_126035655.1	F0F1 ATP synthase subunit gamma	-
EJ066_RS05585	1108239..1109768	-	1530	WP_126035656.1	F0F1 ATP synthase subunit alpha	-
EJ066_RS05590	1109768..1110328	-	561	WP_126035659.1	F0F1 ATP synthase subunit delta	-
EJ066_RS05595	1110574..1110969	-	396	WP_126035661.1	DUF4345 family protein	-
EJ066_RS05600	1111066..1111761	-	696	WP_126035666.1	aspartate/glutamate racemase family protein	-
EJ066_RS05605	1111827..1112069	+	243	WP_126035668.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
EJ066_RS05610	1112066..1112518	+	453	WP_126035670.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJ066_RS05615	1112531..1113473	-	943	Protein_1084	IS630 family transposase	-
EJ066_RS05620	1113571..1115754	-	2184	WP_126035672.1	primosomal protein N'	-
EJ066_RS05625	1115842..1116495	+	654	WP_126035674.1	fructose-6-phosphate aldolase	-
EJ066_RS05630	1116585..1117172	-	588	WP_126035676.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1112531..1113004	473

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-135)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-47)

Sequences

Toxin

Protein

• Length: 151 a.a.
• Molecular weight: 16274.55 Da
• Isoelectric Point: 7.2066

>T116055 WP_126035670.1 NZ_CP034443:1112066-1112518 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
MNGYLLDTSVLSVLAPDRAGLSPTFQAWLSRTGRRQTWYIPAIAAAEVQKGVAKLRRAGGAQRAERLERWLDGLLAEFDE
RVLSIGSAIARRAGEMEDAAIARGRSPGLPDILIAATALEHDLTILTANNRHFEALKVANLNPFGTEPEA

Nucleotide

• Length: 453 bp

>T116055 NZ_CP034443:1112066-1112518 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
TTGAACGGGTACCTGCTCGACACTTCTGTGCTGTCGGTACTCGCGCCTGATCGTGCAGGGCTTTCGCCTACCTTTCAGGC
TTGGCTGTCACGGACCGGCAGACGCCAAACTTGGTATATTCCGGCTATAGCCGCTGCTGAAGTCCAGAAAGGCGTTGCCA
AGTTGAGGCGCGCCGGCGGCGCCCAGCGAGCCGAGCGCCTTGAGCGATGGCTTGACGGCCTACTGGCTGAATTCGATGAG
CGAGTCTTGTCAATAGGCTCCGCCATCGCCCGACGAGCAGGAGAAATGGAAGACGCGGCGATCGCTCGTGGGCGTAGCCC
TGGCCTACCGGACATTTTGATCGCCGCAACTGCTCTTGAGCACGACCTTACGATCTTGACTGCCAACAACCGTCATTTCG
AGGCCCTCAAGGTCGCAAACCTCAACCCATTTGGTACAGAACCCGAAGCCTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8956.16 Da
• Isoelectric Point: 5.0644

>AT116055 WP_126035668.1 NZ_CP034443:1111827-1112069 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
MKTMQVREAKASFSALVEAAENGQPTIITKHGKPAAAVVPIAEARKLYPDRNDDFIDFLLTYPGGIELERNQSTLREYDF

Nucleotide

• Length: 243 bp

>AT116055 NZ_CP034443:1111827-1112069 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
ATGAAGACAATGCAGGTACGAGAGGCAAAGGCGAGCTTTTCTGCGCTTGTGGAGGCGGCGGAGAATGGCCAGCCTACGAT
TATTACCAAGCATGGCAAACCTGCCGCGGCGGTCGTGCCGATCGCAGAGGCGCGAAAGCTCTACCCAGATCGTAACGATG
ATTTTATTGACTTCCTTCTTACTTACCCAGGCGGCATCGAGCTTGAACGAAATCAATCGACGCTCCGGGAATATGACTTT
TGA