Detailed information of TA system TA116054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 755175..755848
• Replicon: chromosome
• Accession: NZ_CP034443
• Organism: Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: EJ066_RS03745
• Protein ID: WP_126035021.1
• Coordinates: 755426..755848 (+)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: EJ066_RS03740
• Protein ID: WP_126035019.1
• Coordinates: 755175..755429 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJ066_RS03700	750447..751172	+	726	WP_126035010.1	YdcF family protein	-
EJ066_RS03705	751192..751800	-	609	WP_126035011.1	pyridoxamine 5'-phosphate oxidase family protein	-
EJ066_RS03710	751813..752283	-	471	WP_065009936.1	DUF1348 family protein	-
EJ066_RS03715	752319..752870	-	552	WP_126035013.1	carboxymuconolactone decarboxylase family protein	-
EJ066_RS03720	752978..753889	+	912	WP_126035015.1	LysR family transcriptional regulator	-
EJ066_RS03725	753998..754792	+	795	WP_126035017.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
EJ066_RS03730	754819..754956	-	138	Protein_721	LysE family translocator	-
EJ066_RS03740	755175..755429	+	255	WP_126035019.1	plasmid stabilization protein	Antitoxin
EJ066_RS03745	755426..755848	+	423	WP_126035021.1	type II toxin-antitoxin system VapC family toxin	Toxin
EJ066_RS03750	755929..756117	+	189	WP_126035023.1	hypothetical protein	-
EJ066_RS03755	756327..756944	-	618	WP_126035025.1	LysE family translocator	-
EJ066_RS03760	757088..758035	-	948	WP_126035027.1	aldo/keto reductase	-
EJ066_RS03765	758188..759156	-	969	WP_189644425.1	helix-turn-helix domain-containing protein	-
EJ066_RS03770	759331..760311	+	981	WP_189644426.1	zinc-binding dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-122)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 14706.81 Da
• Isoelectric Point: 5.5423

>T116054 WP_126035021.1 NZ_CP034443:755426-755848 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
MIVLDTNVVSEAMKPEPAPIVRGWLDEQAAETLYLSSVTIAELLFGTGALPDGRRKQKLATTLDGLLGLFDGRILPFDTD
AARHYADLAVAAHNAGKGFPTPNGYIAAIATAHGFAVATRDASAFIAAGVPVIDPWKPGH

Nucleotide

• Length: 423 bp

>T116054 NZ_CP034443:755426-755848 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
ATGATCGTTCTGGACACGAATGTCGTCTCCGAGGCGATGAAGCCAGAGCCGGCCCCCATCGTTCGCGGCTGGCTGGACGA
GCAGGCTGCTGAAACCCTCTATCTCTCCAGCGTGACCATCGCCGAACTGCTGTTCGGCACCGGTGCGCTACCTGATGGGC
GACGCAAGCAGAAGCTCGCGACGACGCTAGACGGGCTTCTCGGCCTCTTCGACGGCAGAATCCTCCCTTTCGATACAGAT
GCCGCCCGTCATTATGCCGACCTGGCCGTCGCAGCCCACAACGCGGGCAAGGGTTTTCCCACGCCCAACGGATATATCGC
GGCCATCGCGACGGCCCACGGCTTCGCGGTTGCGACCCGCGACGCCAGTGCCTTCATTGCGGCAGGCGTCCCGGTGATTG
ACCCTTGGAAGCCCGGACACTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9110.23 Da
• Isoelectric Point: 6.2207

>AT116054 WP_126035019.1 NZ_CP034443:755175-755429 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
MPAVTIRNLSEEAHRALKVRAAHHGRSTEAEIRDILEAAVRPANRIRIGSALSALSRDAGLTNADFEALEEGCDRTPATP
MSFE

Nucleotide

• Length: 255 bp

>AT116054 NZ_CP034443:755175-755429 [Mesorhizobium sp. M9A.F.Ca.ET.002.03.1.2]
ATGCCCGCCGTCACCATTCGTAACCTGTCCGAGGAAGCACACCGCGCCCTCAAGGTGCGCGCGGCCCATCATGGTCGTAG
CACCGAGGCCGAAATACGCGACATTCTGGAAGCGGCGGTTCGCCCCGCCAACCGCATTCGTATCGGCTCCGCCTTGTCGG
CGTTGAGCCGCGACGCCGGGCTGACCAATGCCGATTTCGAGGCGCTGGAAGAGGGCTGCGACAGGACGCCCGCCACGCCG
ATGAGCTTCGAATGA