Detailed information of TA system TA116044

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2084219..2084748
• Replicon: chromosome
• Accession: NZ_CP034441
• Organism: Staphylococcus aureus strain PMB 81-4

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: EJN89_RS10690
• Protein ID: WP_000621175.1
• Coordinates: 2084386..2084748 (+)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: EJN89_RS10685
• Protein ID: WP_000948331.1
• Coordinates: 2084219..2084389 (+)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EJN89_RS10655	2079255..2079815	+	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-
EJN89_RS10660	2080024..2080503	+	480	WP_001287078.1	hypothetical protein	-
EJN89_RS10665	2080496..2082079	+	1584	WP_001294626.1	PH domain-containing protein	-
EJN89_RS10670	2082066..2082557	+	492	WP_001205912.1	PH domain-containing protein	-
EJN89_RS10675	2082561..2082920	+	360	WP_000581197.1	holo-ACP synthase	-
EJN89_RS10680	2082986..2084134	+	1149	WP_001281154.1	alanine racemase	-
EJN89_RS10685	2084219..2084389	+	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
EJN89_RS10690	2084386..2084748	+	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EJN89_RS10700	2085097..2086098	+	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
EJN89_RS10705	2086217..2086543	+	327	WP_001052491.1	anti-sigma factor antagonist	-
EJN89_RS10710	2086545..2087024	+	480	WP_001190830.1	anti-sigma B factor RsbW	-
EJN89_RS10715	2086999..2087769	+	771	WP_071624271.1	RNA polymerase sigma factor SigB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T116044 WP_000621175.1 NZ_CP034441:2084386-2084748 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T116044 NZ_CP034441:2084386-2084748 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT116044 WP_000948331.1 NZ_CP034441:2084219-2084389 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT116044 NZ_CP034441:2084219-2084389 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1